MicroRNA-17-5p通过靶向PTEN信号诱导卵巢癌细胞耐药和侵袭。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2015-10-22 eCollection Date: 2015-12-01 DOI:10.1186/s40709-015-0035-2

Ying Fang, Changyan Xu, Yan Fu

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引用次数: 2

摘要

背景:miR-17-5p在卵巢癌细胞中过表达，这些细胞用紫杉醇处理。MTT法测定卵巢癌细胞增殖情况。采用Caspase-Glo3/7和TUNEL检测miR-17-5p对紫杉醇诱导的卵巢癌细胞凋亡的影响。采用BD矩阵法分析卵巢癌细胞的迁移和侵袭。Western blot检测卵巢癌细胞中凋亡蛋白和上皮间质转化标志物的表达。结果:过表达miR-17-5p后，卵巢癌细胞存活率提高。miR-17-5p过表达的卵巢癌细胞凋亡减少。miR-17-5p表达改变影响卵巢癌细胞的迁移和侵袭。过表达miR-17-5p改变EMT标志物的表达。miR-17-5p在卵巢癌细胞中通过下调PTEN激活AKT。结论:我们的研究结果表明miR-17-5p可能是潜在的分子治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

MicroRNA-17-5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling.

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MicroRNA-17-5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling.

Background: The miR-17-5p was overexpressed in ovarian cancer cells, and those cells were treated with paclitaxel. The proliferation of ovarian cancer cells was assessed by MTT assay. The Caspase-Glo3/7 and TUNEL assay were used to examine the effect of miR-17-5p on paclitaxel-induced apoptosis in ovarian cancer cells. The migration and invasion of ovarian cancer cells were analyzed by BD matrigel assays. Western blot was performed to evaluate the expression of apoptotic proteins and epithelial-mesenchymal transition markers in ovarian cancer cells.

Results: The survival rate of ovarian cancer cells was increased after overexpression of miR-17-5p. The apoptosis decreased in miR-17-5p overexpressed ovarian cancer cells. Altered miR-17-5p expression affected migration and invasion of ovarian cancer cells. The overexpression of miR-17-5p altered the expression of EMT markers. miR-17-5p activates AKT by downregulation of PTEN in ovarian cancer cells.

Conclusion: Our results indicate that miR-17-5p might serve as potential molecular therapeutic target.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.