古隐菌属新种phocisimile的进一步研究。

Journal of veterinary medicine Pub Date : 2014-01-01 Epub Date: 2014-02-06 DOI:10.1155/2014/923592
Osama Sammra, Anna Balbutskaya, Muaz Hijazin, Samy Nagib, Jörg Alber, Christoph Lämmler, Amir Abdulmawjood, Ellen Prenger-Berninghoff, Markus Timke, Markus Kostrzewa, Ursula Siebert
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引用次数: 9

摘要

通过表型特性、基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)、基因组靶点16S rDNA和16S- 23s rDNA基因间隔区以及rpoB和gap基因的测序,对从一只港海豹阴道拭子中分离到的a . phocisimile型菌株2698(T)和从4只港海豹中分离到的另外4株phocisimile型菌株进行了可靠的鉴定。本研究中所研究的phocisimile菌株与其他几种细菌一起分离,表明phocisimile的致病重要性尚不清楚。然而,利用MALDI-TOF质谱检测肽谱以及所提出的表型和基因型方法可能有助于未来鉴定附子蓟。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

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