PIXSIC:一种自由运动大鼠的无线脑内放射敏感探针。

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular Imaging Pub Date : 2015-01-01

Laure Balasse, Julia Maerk, Frédéric Pain, Aurelie Genoux, Sylvain Fieux, Françoise Lefebvre, Christian Morel, Pascale Gisquet-Verrier, Philippe Lanièce, Luc Zimmer

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引用次数: 0

摘要

本研究的目的是证明无线像素化β+敏感脑内探针(PIXSIC)在清醒和自由活动的啮齿动物体内正电子发射断层扫描(PET)放射药理学中的潜力。注射放射性示踪剂后，在麻醉和清醒大鼠中测量[(11)C]氯氯pride与D2多巴胺受体的结合。20分钟后冷注射氯氯pride评估竞争性结合。该装置能以高时空分辨率准确监测自由运动的啮齿动物的PET配体结合情况。在纹状体和小脑中获得了可重复的时间-活动曲线。在清醒的动物中观察到明显较低的[(11)C]raclopride示踪剂特异性结合。这些初步结果为PET示踪剂在自由活动的啮齿动物体内的药代动力学测量铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

本刊更多论文

PIXSIC: A Wireless Intracerebral Radiosensitive Probe in Freely Moving Rats.

The aim of this study was to demonstrate the potential of a wireless pixelated β+-sensitive intracerebral probe (PIXSIC) for in vivo positron emission tomographic (PET) radiopharmacology in awake and freely moving rodents. The binding of [(11)C]raclopride to D2 dopamine receptors was measured in anesthetized and awake rats following injection of the radiotracer. Competitive binding was assessed with a cold raclopride injection 20 minutes later. The device can accurately monitor binding of PET ligands in freely moving rodents with a high spatiotemporal resolution. Reproducible time-activity curves were obtained for pixels throughout the striatum and cerebellum. A significantly lower [(11)C]raclopride tracer-specific binding was observed in awake animals. These first results pave the way for PET tracer pharmacokinetics measurements in freely moving rodents.

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来源期刊

Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology

自引率

3.60%

发文量

期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.