{"title":"罗得西亚背犬脊等位基因拷贝数诊断测试的开发和验证。","authors":"Jennifer Turner Waldo, Kasandra Santana Diaz","doi":"10.1186/s40575-015-0013-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele.</p><p><strong>Results: </strong>A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally.</p><p><strong>Conclusions: </strong>This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.</p>","PeriodicalId":91060,"journal":{"name":"Canine genetics and epidemiology","volume":"2 ","pages":"2"},"PeriodicalIF":0.0000,"publicationDate":"2015-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40575-015-0013-x","citationCount":"3","resultStr":"{\"title\":\"Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs.\",\"authors\":\"Jennifer Turner Waldo, Kasandra Santana Diaz\",\"doi\":\"10.1186/s40575-015-0013-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele.</p><p><strong>Results: </strong>A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally.</p><p><strong>Conclusions: </strong>This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.</p>\",\"PeriodicalId\":91060,\"journal\":{\"name\":\"Canine genetics and epidemiology\",\"volume\":\"2 \",\"pages\":\"2\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-01-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1186/s40575-015-0013-x\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canine genetics and epidemiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s40575-015-0013-x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2015/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canine genetics and epidemiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s40575-015-0013-x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2015/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs.
Background: The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele.
Results: A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally.
Conclusions: This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.
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