克服酵母 Pichia pastoris 蛋白质表达中的障碍：Pichia 领域领军人物访谈录》（Overcoming Obstacles in the Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field）。
Zoe Ingram, Abha Patkar, Dahoon Oh, Kevin K Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P Lin-Cereghino
{"title":"克服酵母 Pichia pastoris 蛋白质表达中的障碍：Pichia 领域领军人物访谈录》（Overcoming Obstacles in the Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field）。","authors":"Zoe Ingram, Abha Patkar, Dahoon Oh, Kevin K Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P Lin-Cereghino","doi":"10.56031/2576-215x.1010","DOIUrl":null,"url":null,"abstract":"<p><p>The yeast <i>Pichia pastoris</i> (also known as <i>Komagataella pastoris</i>) has been used for over 30 years to produce thousands of valuable, heterologous proteins, such as insulin to treat diabetes and antibodies to prevent migraine headaches. Despite its success, there are some common, stubborn problems encountered by research scientists when they try to use the yeast to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, nine experts in <i>P. pastoris</i> protein expression field were interviewed to create a written review and video (https://www.youtube.com/watch?v=Q1oD6k8CdG8). This review describes how each respected scientist addressed a specific challenge, such as identifying high expression strains, improving secretion efficiency and decreasing hyperglycosylation. Their perspective and practical advice can be a tool to help empower others to express challenging proteins in this popular recombinant host.</p>","PeriodicalId":91036,"journal":{"name":"Pacific journal of health (Stockton, Calif.)","volume":"4 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536841/pdf/nihms-1837807.pdf","citationCount":"0","resultStr":"{\"title\":\"Overcoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field.\",\"authors\":\"Zoe Ingram, Abha Patkar, Dahoon Oh, Kevin K Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P Lin-Cereghino\",\"doi\":\"10.56031/2576-215x.1010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The yeast <i>Pichia pastoris</i> (also known as <i>Komagataella pastoris</i>) has been used for over 30 years to produce thousands of valuable, heterologous proteins, such as insulin to treat diabetes and antibodies to prevent migraine headaches. Despite its success, there are some common, stubborn problems encountered by research scientists when they try to use the yeast to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, nine experts in <i>P. pastoris</i> protein expression field were interviewed to create a written review and video (https://www.youtube.com/watch?v=Q1oD6k8CdG8). This review describes how each respected scientist addressed a specific challenge, such as identifying high expression strains, improving secretion efficiency and decreasing hyperglycosylation. Their perspective and practical advice can be a tool to help empower others to express challenging proteins in this popular recombinant host.</p>\",\"PeriodicalId\":91036,\"journal\":{\"name\":\"Pacific journal of health (Stockton, Calif.)\",\"volume\":\"4 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536841/pdf/nihms-1837807.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pacific journal of health (Stockton, Calif.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.56031/2576-215x.1010\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pacific journal of health (Stockton, Calif.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.56031/2576-215x.1010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Overcoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field.
The yeast Pichia pastoris (also known as Komagataella pastoris) has been used for over 30 years to produce thousands of valuable, heterologous proteins, such as insulin to treat diabetes and antibodies to prevent migraine headaches. Despite its success, there are some common, stubborn problems encountered by research scientists when they try to use the yeast to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, nine experts in P. pastoris protein expression field were interviewed to create a written review and video (https://www.youtube.com/watch?v=Q1oD6k8CdG8). This review describes how each respected scientist addressed a specific challenge, such as identifying high expression strains, improving secretion efficiency and decreasing hyperglycosylation. Their perspective and practical advice can be a tool to help empower others to express challenging proteins in this popular recombinant host.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
