Transgelin-2参与丹参酮- iia诱导的结直肠癌细胞凋亡

IF 2.6 4区 医学 Q3 CELL BIOLOGY
Analytical Cellular Pathology Pub Date : 2022-09-27 eCollection Date: 2022-01-01 DOI:10.1155/2022/9358583
Yingru Zhang, Yiyang Zhao, Jingwen Liu, Chunpu Li, Ying Feng, Shasha Jiang, Xiaoting Sun, Xueqing Hu, Yan Wang
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引用次数: 0

摘要

丹参酮IIA (Tanshinone IIA)是丹参脂溶性成分中的主要活性成分。我们之前的研究已经令人信服地证明了TanIIA是一种有效的抗人结直肠癌细胞的药物。为了进一步证明TanIIA对CRC的作用,我们在体内和体外对其进行了探索性研究。结果表明,TanIIA在抑制肿瘤生长方面明显优于对照组,并延长了患者的生存时间。肿瘤细胞活力和增殖随TanIIA浓度和时间的变化而下降。我们发现TanIIA改变了结直肠癌细胞骨架的形态,并能明显诱导结直肠癌细胞凋亡,阻滞结直肠癌细胞进入G0/G1期。TanIIA还增加了p38MAPK的磷酸化,上调了ATF-2的表达,下调了Transgelin-2的表达,这可以被p38MAPK特异性抑制剂SB203580逆转。我们的研究结果表明,TanIIA可以通过p38MAPK信号通路诱导结直肠癌细胞凋亡,阻断G0/G1期参与下调Transgelin-2表达的细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transgelin-2 Involves in the Apoptosis of Colorectal Cancer Cells Induced by Tanshinone-IIA.

Transgelin-2 Involves in the Apoptosis of Colorectal Cancer Cells Induced by Tanshinone-IIA.

Transgelin-2 Involves in the Apoptosis of Colorectal Cancer Cells Induced by Tanshinone-IIA.

Transgelin-2 Involves in the Apoptosis of Colorectal Cancer Cells Induced by Tanshinone-IIA.

Tanshinone IIA (TanIIA) is the main active ingredient in the fat-soluble components isolated from Salvia miltiorrhiza Bunge. Our previous studies have convincingly proved that TanIIA is an effective drug against human colorectal carcinoma cells. In order to further demonstrate the effect of TanIIA on CRC, we carried out exploratory research about it in vivo and in vitro. The results demonstrated that TanIIA were observably more effective than control group in preventing tumor growth, and it has increased the survival time. Cancer cells viability and proliferation were accompanied by concentration and time dependent decline reached with TanIIA. We found that TanIIA altered the morphology of cytoskeleton and it could obviously induce apoptosis of colorectal cancer cells and block the cells in the G0/G1 phase. TanIIA also increased phosphorylation of p38MAPK, upregulated ATF-2 expression and downregulated Transgelin-2 expression, which could be reversed by SB203580, a p38MAPK-specific inhibitor. Our results suggested that TanIIA could induce apoptosis of colorectal cancer and block the cells in G0/G1 phase involved in downregulating the expression of Transgelin-2 through p38MAPK signal pathway.

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来源期刊
Analytical Cellular Pathology
Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY
CiteScore
4.90
自引率
3.10%
发文量
70
审稿时长
16 weeks
期刊介绍: Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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