Karine de Mattos, Camilo Andrés Pena-Bello, Karine Campagnolo, Gabriella Borba de Oliveira, Elvis Ticiani, César Augusto Pinzón-Osorio, Ana Laura da Silva Feijó, Higor da Silva Ferreira, José Luiz Rodrigues, Marcelo Bertolini, Alceu Mezzallira, Eduardo de Souza Ribeiro
{"title":"在培养基中添加β-巯基乙醇可提高体外培养的牛胚胎的低温耐受性。","authors":"Karine de Mattos, Camilo Andrés Pena-Bello, Karine Campagnolo, Gabriella Borba de Oliveira, Elvis Ticiani, César Augusto Pinzón-Osorio, Ana Laura da Silva Feijó, Higor da Silva Ferreira, José Luiz Rodrigues, Marcelo Bertolini, Alceu Mezzallira, Eduardo de Souza Ribeiro","doi":"10.1017/S0967199422000338","DOIUrl":null,"url":null,"abstract":"<p><p>The objective of this study was to investigate the effects of adding β-mercaptoethanol (βME) to culture medium of bovine <i>in vitro</i>-produced (IVP) embryos prior to or after vitrification on embryo development and cryotolerance. In Experiment I, Day-7 IVP blastocysts were vitrified and, after warming, cultured in medium containing 0, 50 or 100 μM βME for 72 h. Embryos cultured in 100 μM βME attained higher hatching rates (66.7%) than those culture in 0 (47.7%) and 50 (52.4%) μM βME. In Experiment II, IVP embryos were <i>in vitro</i>-cultured (IVC) to the blastocyst stage in 0 (control) or 100 μM βME, followed by vitrification. After warming, embryos were cultured for 72 h (post-warming culture, PWC) in 0 (control) or 100 μM βME, in a 2 × 2 factorial design: (i) CTRL-CTRL, control IVC and control PWC; (ii) CTRL-βME, control IVC and βME-supplemented PWC; (iii) βME-CTRL, βME-supplemented IVC and control PWC; or (iv) βME-βME, βME-supplemented IVC and βME-supplemented PWC. βME during IVC reduced embryo development (28.0% vs. 43.8%) but, following vitrification, higher re-expansion rates were seen in βME-CTRL (84.0%) and βME-βME (87.5%) than in CTRL-CTRL (71.0%) and CTRL-βME (73.1%). Hatching rates were higher in CTRL-βME (58.1%) and βME-βME (63.8%) than in CTRL-CTRL (36.6%) and βME-CTRL (42.0%). Total cell number in hatched blastocysts was higher in βME-βME (181.2 ± 7.4 cells) than CTRL-CTRL (139.0 ± 9.9 cells). Adding βME to the IVC medium reduced development but increased cryotolerance, whereas adding βME to the PWC medium improved embryo survival, hatching rates, and total cell numbers.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"β-Mercaptoethanol in culture medium improves cryotolerance of <i>in vitro</i>-produced bovine embryos.\",\"authors\":\"Karine de Mattos, Camilo Andrés Pena-Bello, Karine Campagnolo, Gabriella Borba de Oliveira, Elvis Ticiani, César Augusto Pinzón-Osorio, Ana Laura da Silva Feijó, Higor da Silva Ferreira, José Luiz Rodrigues, Marcelo Bertolini, Alceu Mezzallira, Eduardo de Souza Ribeiro\",\"doi\":\"10.1017/S0967199422000338\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The objective of this study was to investigate the effects of adding β-mercaptoethanol (βME) to culture medium of bovine <i>in vitro</i>-produced (IVP) embryos prior to or after vitrification on embryo development and cryotolerance. In Experiment I, Day-7 IVP blastocysts were vitrified and, after warming, cultured in medium containing 0, 50 or 100 μM βME for 72 h. Embryos cultured in 100 μM βME attained higher hatching rates (66.7%) than those culture in 0 (47.7%) and 50 (52.4%) μM βME. In Experiment II, IVP embryos were <i>in vitro</i>-cultured (IVC) to the blastocyst stage in 0 (control) or 100 μM βME, followed by vitrification. After warming, embryos were cultured for 72 h (post-warming culture, PWC) in 0 (control) or 100 μM βME, in a 2 × 2 factorial design: (i) CTRL-CTRL, control IVC and control PWC; (ii) CTRL-βME, control IVC and βME-supplemented PWC; (iii) βME-CTRL, βME-supplemented IVC and control PWC; or (iv) βME-βME, βME-supplemented IVC and βME-supplemented PWC. βME during IVC reduced embryo development (28.0% vs. 43.8%) but, following vitrification, higher re-expansion rates were seen in βME-CTRL (84.0%) and βME-βME (87.5%) than in CTRL-CTRL (71.0%) and CTRL-βME (73.1%). Hatching rates were higher in CTRL-βME (58.1%) and βME-βME (63.8%) than in CTRL-CTRL (36.6%) and βME-CTRL (42.0%). Total cell number in hatched blastocysts was higher in βME-βME (181.2 ± 7.4 cells) than CTRL-CTRL (139.0 ± 9.9 cells). Adding βME to the IVC medium reduced development but increased cryotolerance, whereas adding βME to the PWC medium improved embryo survival, hatching rates, and total cell numbers.</p>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1017/S0967199422000338\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/9/23 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1017/S0967199422000338","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/9/23 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
β-Mercaptoethanol in culture medium improves cryotolerance of in vitro-produced bovine embryos.
The objective of this study was to investigate the effects of adding β-mercaptoethanol (βME) to culture medium of bovine in vitro-produced (IVP) embryos prior to or after vitrification on embryo development and cryotolerance. In Experiment I, Day-7 IVP blastocysts were vitrified and, after warming, cultured in medium containing 0, 50 or 100 μM βME for 72 h. Embryos cultured in 100 μM βME attained higher hatching rates (66.7%) than those culture in 0 (47.7%) and 50 (52.4%) μM βME. In Experiment II, IVP embryos were in vitro-cultured (IVC) to the blastocyst stage in 0 (control) or 100 μM βME, followed by vitrification. After warming, embryos were cultured for 72 h (post-warming culture, PWC) in 0 (control) or 100 μM βME, in a 2 × 2 factorial design: (i) CTRL-CTRL, control IVC and control PWC; (ii) CTRL-βME, control IVC and βME-supplemented PWC; (iii) βME-CTRL, βME-supplemented IVC and control PWC; or (iv) βME-βME, βME-supplemented IVC and βME-supplemented PWC. βME during IVC reduced embryo development (28.0% vs. 43.8%) but, following vitrification, higher re-expansion rates were seen in βME-CTRL (84.0%) and βME-βME (87.5%) than in CTRL-CTRL (71.0%) and CTRL-βME (73.1%). Hatching rates were higher in CTRL-βME (58.1%) and βME-βME (63.8%) than in CTRL-CTRL (36.6%) and βME-CTRL (42.0%). Total cell number in hatched blastocysts was higher in βME-βME (181.2 ± 7.4 cells) than CTRL-CTRL (139.0 ± 9.9 cells). Adding βME to the IVC medium reduced development but increased cryotolerance, whereas adding βME to the PWC medium improved embryo survival, hatching rates, and total cell numbers.
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Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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