新生儿和成人巨细胞病毒感染小鼠模型。

Current Protocols Pub Date : 2022-09-01 DOI:10.1002/cpz1.537

Ilija Brizić, Berislav Lisnić, Fran Krstanović, Wolfram Brune, Hartmut Hengel, Stipan Jonjić

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Mouse Models for Cytomegalovirus Infections in Newborns and Adults.

This article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay. © 2022 Wiley Periodicals LLC.

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Current Protocols

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