[一种新的荧光模拟物，用于研究细胞培养中anandamide运输]。

Bioorganicheskaia khimiia Pub Date : 2014-03-01

N M Gretskaia, M G Akimov, V V Bezuglov

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引用次数: 0

摘要

首次制备了一种新的含BODIPY®- fl -荧光基团的阿纳达胺荧光类似物，通过2,2'-(乙二氧基)-二(乙二胺)连接到花生四烯酸。在大鼠胶质瘤C6细胞中，荧光类似物被证明是细胞anandamide摄取系统的底物(Km 4.5±0.9µM, Vmax 20±1 amol/(min x细胞))。

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[A new fluorescent analogue for the investigation of anandamide transport in cell cultures].

For the first time a new fluorescent analogue of anadamide incorporating BODIPY®-FL-fluorophore, attached to arachidonic acid via 2,2'-(ethylenedioxy)-bis(ethylenediamine), was prepared. Using rat glioma C6 cells it was demonstrated that the fluorescent analogue is a substrate of the cellular anandamide uptake system (Km 4.5 ± 0.9 µM, Vmax 20 ± 1 amol/(min x cell)).

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Bioorganicheskaia khimiia

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