利什曼原虫的硒代半胱氨酸tRNA基因由RNA聚合酶II和RNA聚合酶III转录。

Eukaryotic Cell Pub Date : 2015-03-01 Epub Date: 2014-12-29 DOI:10.1128/EC.00239-14
Norma E Padilla-Mejía, Luis E Florencio-Martínez, Rodrigo Moreno-Campos, Juan C Vizuet-de-Rueda, Ana M Cevallos, Rosaura Hernández-Rivas, Rebeca Manning-Cela, Santiago Martínez-Calvillo
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引用次数: 9

摘要

真核trna由RNA聚合酶III (Pol III)转录,包含盒子A和B作为内部启动子元件。一个例外是硒代半胱氨酸(Sec) tRNA (tRNA-Sec),在脊椎动物中,其转录是由一个内部框B和三个外基因序列指导的。本文报道了原生动物利什曼原虫tRNA-Sec基因的转录分析。这种生物具有不同寻常的基因表达机制,包括Pol II多顺反子转录和mrna的反式剪接成熟,这一过程将39个核苷酸的小外显子连接到所有mrna的5'端。在L. major中,tRNA-Sec是由插入Pol II多反式电子单元的单个基因编码的,而大多数trna是聚集在多反式电子单元的边界上。cDNA末端的5′快速扩增和逆转录- pcr实验表明,一些tRNA-Sec转录本在5′端含有miniexon,在3′端含有poly(a)尾,这表明tRNA-Sec基因是由Pol II多顺反转录并经过反式剪接和聚腺苷化处理的,正如最近报道的在相关寄生虫布鲁氏锥虫中的tRNA-Sec基因。然而,RNA聚合酶抑制剂和先前紫外线照射的细胞的核运行试验表明,L. major中的tRNA-Sec基因也被Pol III转录。因此,我们的结果表明,RNA聚合酶在利什曼原虫体内并不是绝对的特异性,正如最近在其他真核生物中发现的那样。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III.

The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III.

The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III.

The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III.

Eukaryotic tRNAs, transcribed by RNA polymerase III (Pol III), contain boxes A and B as internal promoter elements. One exception is the selenocysteine (Sec) tRNA (tRNA-Sec), whose transcription is directed by an internal box B and three extragenic sequences in vertebrates. Here we report on the transcriptional analysis of the tRNA-Sec gene in the protozoan parasite Leishmania major. This organism has unusual mechanisms of gene expression, including Pol II polycistronic transcription and maturation of mRNAs by trans splicing, a process that attaches a 39-nucleotide miniexon to the 5' end of all the mRNAs. In L. major, tRNA-Sec is encoded by a single gene inserted into a Pol II polycistronic unit, in contrast to most tRNAs, which are clustered at the boundaries of polycistronic units. 5' rapid amplification of cDNA ends and reverse transcription-PCR experiments showed that some tRNA-Sec transcripts contain the miniexon at the 5' end and a poly(A) tail at the 3' end, indicating that the tRNA-Sec gene is polycistronically transcribed by Pol II and processed by trans splicing and polyadenylation, as was recently reported for the tRNA-Sec genes in the related parasite Trypanosoma brucei. However, nuclear run-on assays with RNA polymerase inhibitors and with cells that were previously UV irradiated showed that the tRNA-Sec gene in L. major is also transcribed by Pol III. Thus, our results indicate that RNA polymerase specificity in Leishmania is not absolute in vivo, as has recently been found in other eukaryotes.

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来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
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1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
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