FilmArray法与室内实时荧光定量PCR检测呼吸道感染的比较。

Maria E Andersson, Sigvard Olofsson, Magnus Lindh
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引用次数: 50

摘要

最近,能够检测几乎所有可能引起急性呼吸道感染的微生物病原体的分子方法已经被引入。FilmArray Respiratory Panel检测将核酸提取、巢式扩增和检测集成在预装有检测17种病毒和3种细菌所需的所有试剂的反应袋中,与内部实时PCR检测这些试剂进行8次平行扩增进行比较。当128个临床样本代表18种药物被两种方法分析时,一致性非常好,kappa值在0.54和1.0之间。不一致主要见于腺病毒,但在使用FilmArray 1.7版本时没有发现。结果表明,这些检测方法检测范围广泛,具有相似的性能。FilmArray在大约1小时后提供结果,包括≈5分钟的操作时间,并且不需要先进的设备或分子诊断专业知识,使其成为急性呼吸道感染的有用的护理点测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of the FilmArray assay and in-house real-time PCR for detection of respiratory infection.

Recently, molecular methods capable of detecting almost all microbial agents that may cause acute respiratory infection have been introduced. The FilmArray Respiratory Panel assay, which integrates nucleic acid extraction, nested amplification and detection in a reaction pouch preloaded with all reagents required for detection of 17 viruses and 3 bacteria, was compared with an in-house real-time PCR that detects these agents in 8 parallel amplifications. When 128 clinical samples representing 18 of these agents were analysed by both assays the agreement was excellent, with kappa values ranging between 0.54 and 1.0. Discordances were mainly observed for adenovirus, but not when version 1.7 of FilmArray was used. The results show that these assays detect a wide range of pathogens with similar performance. FilmArray provides results after approximately 1 h, including ≈ 5 min hands-on time, and does not require advanced equipment or expertise in molecular diagnostics, making it a useful point-of-care-test for acute respiratory infections.

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