{"title":"体外培养时间对牛生长卵母细胞受精率及卵母细胞周围细胞增殖的影响。","authors":"Weiping Huang, Sung-Sik Kang, Yojiro Yanagawa, Yinghua Yang, Yoshiyuki Takahashi, Masashi Nagano","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The effects of in vitro-growth (IVG) culture duration (12 and 14 days) on the fertilizability and the developmental ability of IVG oocytes matured with pre-maturational culture, and on the proliferation of cells surrounding oocytes during IVG culture were examined. The fertilization and cleavage rates of 12- and 14-day IVG oocytes were similar; however, 14-day IVG oocytes showed lower blastocyst development rate than 12-day IVG oocytes. In addition, the number of cells surrounding oocytes increased until 12 days of IVG, but decreased at 14 days. The results indicated that the extension of IVG period beyond 12 days impaired the proliferation of cells surrounding oocytes, leading to the decrease of developmental ability but not fertilizability of 14-day IVG oocytes.</p>","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of in vitro-growth culture duration on fertilizability of bovine growing oocytes and proliferation of cells surrounding oocytes.\",\"authors\":\"Weiping Huang, Sung-Sik Kang, Yojiro Yanagawa, Yinghua Yang, Yoshiyuki Takahashi, Masashi Nagano\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The effects of in vitro-growth (IVG) culture duration (12 and 14 days) on the fertilizability and the developmental ability of IVG oocytes matured with pre-maturational culture, and on the proliferation of cells surrounding oocytes during IVG culture were examined. The fertilization and cleavage rates of 12- and 14-day IVG oocytes were similar; however, 14-day IVG oocytes showed lower blastocyst development rate than 12-day IVG oocytes. In addition, the number of cells surrounding oocytes increased until 12 days of IVG, but decreased at 14 days. The results indicated that the extension of IVG period beyond 12 days impaired the proliferation of cells surrounding oocytes, leading to the decrease of developmental ability but not fertilizability of 14-day IVG oocytes.</p>\",\"PeriodicalId\":56285,\"journal\":{\"name\":\"Japanese Journal of Veterinary Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2014-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Veterinary Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Veterinary Research","FirstCategoryId":"97","ListUrlMain":"","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Effects of in vitro-growth culture duration on fertilizability of bovine growing oocytes and proliferation of cells surrounding oocytes.
The effects of in vitro-growth (IVG) culture duration (12 and 14 days) on the fertilizability and the developmental ability of IVG oocytes matured with pre-maturational culture, and on the proliferation of cells surrounding oocytes during IVG culture were examined. The fertilization and cleavage rates of 12- and 14-day IVG oocytes were similar; however, 14-day IVG oocytes showed lower blastocyst development rate than 12-day IVG oocytes. In addition, the number of cells surrounding oocytes increased until 12 days of IVG, but decreased at 14 days. The results indicated that the extension of IVG period beyond 12 days impaired the proliferation of cells surrounding oocytes, leading to the decrease of developmental ability but not fertilizability of 14-day IVG oocytes.
期刊介绍：
The Japanese Journal of Veterinary Research (JJVR) quarterly publishes peer-reviewed articles on all aspects of veterinary science. JJVR was originally published as a “University Journal” of veterinary science at Hokkaido University from more than 60 years ago. Currently, JJVR, is Japan’s leading scientific veterinary journal, and provides valuable information for the development of veterinary science by welcoming contributions from researchers worldwide.
JJVR offers online submission for Regular Papers, Short Communications, and Review Articles that are unpublished and not being considered for publication elsewhere. Research areas include:
Anatomy, Physiology, Biochemistry, Pharmacology, Microbiology, Infectious diseases, Parasitology, Laboratory Animal Science and Medicine, Internal Medicine, Surgery, Pathology, Theriogenology, Molecular Medicine, Public Health, Radiation Biology, Toxicology, Wildlife Biology and Medicine, Veterinary Hygiene, The other fields related to veterinary science.
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