An all-at-once 2D CEST by F1-spatial frequency encoding

In this work we present a 2D NMR experiment that provides insight into the full chemical exchange saturation transfer (CEST) network present in a sample. It yields all CEST profiles between any signals in a spectrum at once. The method relies on a combination of slice selective saturation during the preparation period, combined with an inverse read-out gradient applied during the evolution time. The resulting 2D spectrum yields gradient profiles in F1 with dips at the frequencies of signals that show a CEST to the corresponding signal in F2.