{"title":"[RNA干扰TLR4信号通路对Kupffer细胞吞噬的影响]。","authors":"Shu-Fei Zhang, Jing Li, Yin-Lan Liu, Wen-Jun Yang, Yan Luo, Zhen-Jie Zhuang, Qi-Bin Jiao, Jian-Yu Chen, Dong-Xue Bian, Xiao-Jie Ma, Yun-Hao Xun, Ming-Li Zhu, Jun-Ping Shi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells.</p><p><strong>Methods: </strong>RAW2647 mice mononuclear macrophage leukemia cells were observed. The tested group was interfered by Tlr4-mus-1567 RNA which had the best result confirmed by QPCR, cells interfered by Negative Control RNA as NC group, and normal cell as control. We perform the phagocytosis test on each group.</p><p><strong>Results: </strong>The tested group has lower phagocytes percentage than control (17.67% +/- 3.51% vs 32.00% +/- 3.00%, P < 0.01), and lower phagocytic index (46.33% +/- 7.51% vs 82.00% +/- 6.08%, P < 0.01).</p><p><strong>Conclusions: </strong>Decreased phagocytic activity was observed on Kupffer cells by RNA interference.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 5","pages":"322-4"},"PeriodicalIF":0.0000,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[The effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells].\",\"authors\":\"Shu-Fei Zhang, Jing Li, Yin-Lan Liu, Wen-Jun Yang, Yan Luo, Zhen-Jie Zhuang, Qi-Bin Jiao, Jian-Yu Chen, Dong-Xue Bian, Xiao-Jie Ma, Yun-Hao Xun, Ming-Li Zhu, Jun-Ping Shi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells.</p><p><strong>Methods: </strong>RAW2647 mice mononuclear macrophage leukemia cells were observed. The tested group was interfered by Tlr4-mus-1567 RNA which had the best result confirmed by QPCR, cells interfered by Negative Control RNA as NC group, and normal cell as control. We perform the phagocytosis test on each group.</p><p><strong>Results: </strong>The tested group has lower phagocytes percentage than control (17.67% +/- 3.51% vs 32.00% +/- 3.00%, P < 0.01), and lower phagocytic index (46.33% +/- 7.51% vs 82.00% +/- 6.08%, P < 0.01).</p><p><strong>Conclusions: </strong>Decreased phagocytic activity was observed on Kupffer cells by RNA interference.</p>\",\"PeriodicalId\":70973,\"journal\":{\"name\":\"中华实验和临床病毒学杂志\",\"volume\":\"27 5\",\"pages\":\"322-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验和临床病毒学杂志\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验和临床病毒学杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:探讨RNA干扰TLR4信号通路对Kupffer细胞吞噬的影响。方法:观察RAW2647小鼠单核巨噬细胞白血病细胞。实验组用QPCR证实效果最好的tlr4 -mu -1567 RNA干扰，阴性对照RNA干扰的细胞为NC组，正常细胞为对照。对各组进行吞噬试验。结果:实验组吞噬百分率低于对照组(17.67% +/- 3.51% vs 32.00% +/- 3.00%， P < 0.01)，吞噬指数低于对照组(46.33% +/- 7.51% vs 82.00% +/- 6.08%， P < 0.01)。结论:RNA干扰可降低Kupffer细胞的吞噬活性。
[The effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells].
Objective: To investigate the effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells.
Methods: RAW2647 mice mononuclear macrophage leukemia cells were observed. The tested group was interfered by Tlr4-mus-1567 RNA which had the best result confirmed by QPCR, cells interfered by Negative Control RNA as NC group, and normal cell as control. We perform the phagocytosis test on each group.
Results: The tested group has lower phagocytes percentage than control (17.67% +/- 3.51% vs 32.00% +/- 3.00%, P < 0.01), and lower phagocytic index (46.33% +/- 7.51% vs 82.00% +/- 6.08%, P < 0.01).
Conclusions: Decreased phagocytic activity was observed on Kupffer cells by RNA interference.
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