S. Sureshkumar, P. Tamilkumar, N. Senthil, P. Nagarajan, A. U. Thangavelu, M. Raveendran, S. Vellaikumar, K. N. Ganesan, R. Balagopal, G. Vijayalakshmi, V. Shobana
{"title":"玉米低植酸性状的标记辅助选择","authors":"S. Sureshkumar, P. Tamilkumar, N. Senthil, P. Nagarajan, A. U. Thangavelu, M. Raveendran, S. Vellaikumar, K. N. Ganesan, R. Balagopal, G. Vijayalakshmi, V. Shobana","doi":"10.1111/j.1601-5223.2013.00030.x","DOIUrl":null,"url":null,"abstract":"<p>Maize is the third important major food crop. Breeding for low phytate maize genotypes is an effective strategy for decreasing the content of kernel phytic acid (a chelator of cations such as Ca<sup>2+</sup> and Fe<sup>3+</sup>) and thereby increasing the bioavailability of nutritive minerals in human diet and animal feed. Previous studies have established that a mutant plant with a <i>lpa2-2</i> allele accumulates less phytic acid in seeds. Therefore, the marker assisted backcross breeding (MABB), which involves introgression of <i>lpa2-2</i> recessive allele (which confer low phytate trait) from a <i>lpa2-2</i> mutant line into a well-adapted line using backcrosses and selection of lines possessing <i>lpa2-2</i> allele in each backcross population using molecular markers, is an effective strategy for developing low phytate maize. So far, no studies have developed any <i>lpa2-2</i> allele specific molecular markers for this purpose. Here, using backcross and selfed progenies, obtained by crossing low phytate mutant line ‘EC 659418’ (i.e. donor of <i>lpa2-2</i> allele) into agronomically superior line ‘UMI395’, we have validated that a SSR marker ‘umc2230’, located 0.4 cM downstream of <i>lpa2-2</i>, cosegregate, in a Mendelian fashion, with low phytic acid trait. Therefore umc2230 can be dependably used in MABB for the development of low phytate maize.</p>","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2014-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1601-5223.2013.00030.x","citationCount":"20","resultStr":"{\"title\":\"Marker assisted selection of low phytic acid trait in maize (Zea mays L.)\",\"authors\":\"S. Sureshkumar, P. Tamilkumar, N. Senthil, P. Nagarajan, A. U. Thangavelu, M. Raveendran, S. Vellaikumar, K. N. Ganesan, R. Balagopal, G. Vijayalakshmi, V. Shobana\",\"doi\":\"10.1111/j.1601-5223.2013.00030.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Maize is the third important major food crop. Breeding for low phytate maize genotypes is an effective strategy for decreasing the content of kernel phytic acid (a chelator of cations such as Ca<sup>2+</sup> and Fe<sup>3+</sup>) and thereby increasing the bioavailability of nutritive minerals in human diet and animal feed. Previous studies have established that a mutant plant with a <i>lpa2-2</i> allele accumulates less phytic acid in seeds. Therefore, the marker assisted backcross breeding (MABB), which involves introgression of <i>lpa2-2</i> recessive allele (which confer low phytate trait) from a <i>lpa2-2</i> mutant line into a well-adapted line using backcrosses and selection of lines possessing <i>lpa2-2</i> allele in each backcross population using molecular markers, is an effective strategy for developing low phytate maize. So far, no studies have developed any <i>lpa2-2</i> allele specific molecular markers for this purpose. Here, using backcross and selfed progenies, obtained by crossing low phytate mutant line ‘EC 659418’ (i.e. donor of <i>lpa2-2</i> allele) into agronomically superior line ‘UMI395’, we have validated that a SSR marker ‘umc2230’, located 0.4 cM downstream of <i>lpa2-2</i>, cosegregate, in a Mendelian fashion, with low phytic acid trait. Therefore umc2230 can be dependably used in MABB for the development of low phytate maize.</p>\",\"PeriodicalId\":55057,\"journal\":{\"name\":\"Hereditas\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2014-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1601-5223.2013.00030.x\",\"citationCount\":\"20\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hereditas\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1601-5223.2013.00030.x\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hereditas","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1601-5223.2013.00030.x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Marker assisted selection of low phytic acid trait in maize (Zea mays L.)
Maize is the third important major food crop. Breeding for low phytate maize genotypes is an effective strategy for decreasing the content of kernel phytic acid (a chelator of cations such as Ca2+ and Fe3+) and thereby increasing the bioavailability of nutritive minerals in human diet and animal feed. Previous studies have established that a mutant plant with a lpa2-2 allele accumulates less phytic acid in seeds. Therefore, the marker assisted backcross breeding (MABB), which involves introgression of lpa2-2 recessive allele (which confer low phytate trait) from a lpa2-2 mutant line into a well-adapted line using backcrosses and selection of lines possessing lpa2-2 allele in each backcross population using molecular markers, is an effective strategy for developing low phytate maize. So far, no studies have developed any lpa2-2 allele specific molecular markers for this purpose. Here, using backcross and selfed progenies, obtained by crossing low phytate mutant line ‘EC 659418’ (i.e. donor of lpa2-2 allele) into agronomically superior line ‘UMI395’, we have validated that a SSR marker ‘umc2230’, located 0.4 cM downstream of lpa2-2, cosegregate, in a Mendelian fashion, with low phytic acid trait. Therefore umc2230 can be dependably used in MABB for the development of low phytate maize.
期刊介绍:
For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.