玻璃质粘连蛋白的纯化

Q3 Biochemistry, Genetics and Molecular Biology

Current Protocols in Cell Biology Pub Date : 2018-04-25 DOI:10.1002/0471143030.cb1006s60

Steven K. Akiyama

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引用次数: 6

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Purification of Vitronectin

This unit describes the purification of extracellular vitronectin from plasma or serum by using heparin-affinity chromatography. First, the plasma is depleted of fibronectin plus other heparin- and Sepharose-binding proteins and treated with urea to activate the heparin-binding activity of vitronectin, which is subsequently bound to a heparin affinity column and eluted. The resulting vitronectin should be ∼98% pure. Curr. Protoc. Cell Biol. 60:10.6.1-10.6.7. © 2013 by John Wiley & Sons, Inc.

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Current Protocols in Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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期刊介绍： Developed by leading scientists in the field, Current Protocols in Cell Biology is an essential reference for researchers who study the relationship between specific molecules and genes and their location, function and structure at the cellular level. Updated every three months in all formats, CPCB is constantly evolving to keep pace with the very latest discoveries and developments.