Tianran Li , Yinhao Ye , Peng Wu, Rengang Luo, Hongyu Zhang, Weiwei Zheng
{"title":"蛋白酶体β3亚基(PSMB3)在背小实蝇(Bactrocera dorsalis)性成熟过程中通过促进卵巢甾体形成来控制雌性生殖","authors":"Tianran Li , Yinhao Ye , Peng Wu, Rengang Luo, Hongyu Zhang, Weiwei Zheng","doi":"10.1016/j.ibmb.2023.103959","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span>Steroid hormone<span> 20-hydroxyecdysone (20E) plays critical roles in reproductive development in dipterans and several other insect species. Ecdysteroidogenesis in the glands of larval or nymphal insects and other arthropods has been extensively studied, but that in the adult </span></span>gonads remains largely unknown. Here we identified a </span>proteasome β3 subunit (</span><em>PSMB3</em><span>) from a highly invasive pest </span><span><em>Bactrocera dorsalis</em></span><span>, and found that this gene was crucial for ecdysone production during female reproduction</span><em>. PSMB3</em> was enriched in the ovary, and it was upregulated during sexual maturation. RNAi-mediated depletion of <em>PSMB3</em><span> resulted in retarded ovarian development and decreased fecundity. Additionally, knockdown of </span><em>PSMB3</em> reduced 20E titer in hemolymph of <em>B. dorsalis</em><span>. Molecularly, RNA sequencing and qPCR validation revealed that </span><em>PSMB3</em> depletion suppressed the expression of 20E biosynthetic genes in the ovary and 20E responsive genes in the ovary and fat body. Furthermore, exogenous 20E rescued the inhibition of the ovarian development caused by <em>PSMB3</em> depletion<em>.</em><span> Taken together, this study provides new insights into the adult reproductive development-related biological processes controlled by </span><em>PSMB3</em>, and proposed a potential eco-friendly control strategy against this notorious agricultural pest.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteasome β3 subunit (PSMB3) controls female reproduction by promoting ecdysteroidogenesis during sexual maturation in Bactrocera dorsalis\",\"authors\":\"Tianran Li , Yinhao Ye , Peng Wu, Rengang Luo, Hongyu Zhang, Weiwei Zheng\",\"doi\":\"10.1016/j.ibmb.2023.103959\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span><span>Steroid hormone<span> 20-hydroxyecdysone (20E) plays critical roles in reproductive development in dipterans and several other insect species. Ecdysteroidogenesis in the glands of larval or nymphal insects and other arthropods has been extensively studied, but that in the adult </span></span>gonads remains largely unknown. Here we identified a </span>proteasome β3 subunit (</span><em>PSMB3</em><span>) from a highly invasive pest </span><span><em>Bactrocera dorsalis</em></span><span>, and found that this gene was crucial for ecdysone production during female reproduction</span><em>. PSMB3</em> was enriched in the ovary, and it was upregulated during sexual maturation. RNAi-mediated depletion of <em>PSMB3</em><span> resulted in retarded ovarian development and decreased fecundity. Additionally, knockdown of </span><em>PSMB3</em> reduced 20E titer in hemolymph of <em>B. dorsalis</em><span>. Molecularly, RNA sequencing and qPCR validation revealed that </span><em>PSMB3</em> depletion suppressed the expression of 20E biosynthetic genes in the ovary and 20E responsive genes in the ovary and fat body. Furthermore, exogenous 20E rescued the inhibition of the ovarian development caused by <em>PSMB3</em> depletion<em>.</em><span> Taken together, this study provides new insights into the adult reproductive development-related biological processes controlled by </span><em>PSMB3</em>, and proposed a potential eco-friendly control strategy against this notorious agricultural pest.</p></div>\",\"PeriodicalId\":330,\"journal\":{\"name\":\"Insect Biochemistry and Molecular Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S096517482300053X\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S096517482300053X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Proteasome β3 subunit (PSMB3) controls female reproduction by promoting ecdysteroidogenesis during sexual maturation in Bactrocera dorsalis
Steroid hormone 20-hydroxyecdysone (20E) plays critical roles in reproductive development in dipterans and several other insect species. Ecdysteroidogenesis in the glands of larval or nymphal insects and other arthropods has been extensively studied, but that in the adult gonads remains largely unknown. Here we identified a proteasome β3 subunit (PSMB3) from a highly invasive pest Bactrocera dorsalis, and found that this gene was crucial for ecdysone production during female reproduction. PSMB3 was enriched in the ovary, and it was upregulated during sexual maturation. RNAi-mediated depletion of PSMB3 resulted in retarded ovarian development and decreased fecundity. Additionally, knockdown of PSMB3 reduced 20E titer in hemolymph of B. dorsalis. Molecularly, RNA sequencing and qPCR validation revealed that PSMB3 depletion suppressed the expression of 20E biosynthetic genes in the ovary and 20E responsive genes in the ovary and fat body. Furthermore, exogenous 20E rescued the inhibition of the ovarian development caused by PSMB3 depletion. Taken together, this study provides new insights into the adult reproductive development-related biological processes controlled by PSMB3, and proposed a potential eco-friendly control strategy against this notorious agricultural pest.
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.