高龋患者牙髓间充质干细胞的再生潜力。

Q4 Biochemistry, Genetics and Molecular Biology
Journal of Stem Cells Pub Date : 2013-01-01
Ramesh Rajendran, Sushruth Gopal, Huda Masood, Purushottam Vivek, Kaushik Deb
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引用次数: 0

摘要

众所周知,牙髓中含有负责修复牙本质的干细胞或牙本质原体细胞。人脱落乳牙(SHED)牙髓干细胞是一种具有广泛增殖和多潜能或多谱系分化能力的产后干细胞。这种组织再生的潜力已成为当前牙髓干细胞库的基础。在这里,我们试图开发一种从高龋病患者的牙齿中收集干细胞的方案,这些患者通常被选择性地丢弃。我们用间充质干细胞标记物对干细胞进行了表征,并比较了它们与正常骨髓间充质干细胞(MSCs)在培养、倍增和分化成不同谱系中的潜力。我们观察到牙髓中的MSCs生长更快,倍增时间更短,并且在标准定向分化方案下具有相同的分化到各种谱系的效率。本文证实,废弃的高携带牙齿可以作为再生医学的良好来源,也可以作为MSC和牙髓MSC库的潜在来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regenerative potential of dental pulp mesenchymal stem cells harvested from high caries patient's teeth.

Dental pulp are known to contains stem cells or dentinogenic progenitors that are responsible for dentin repair. Dental pulp Stem cells from Human Exfoliated Deciduous teeth (SHED) represent a population of postnatal stem cells capable of extensive proliferation and multipotential or multilineage differentiations. This potential for tissue regeneration has become the current basis for dental pulp stem cell banking. Here, we have attempted to develop a protocol for harvesting stem cells from patients with High Caries tooth, which are most often electively discarded. We have characterized the stem cells with mesenchymal stem cell markers and have compared their potential to grow in culture, doubling times, and differentiate into different lineages, with normal bone marrow mesenchymal stem cells (MSCs). We observed that the MSCs from dental pulp grew faster, with lower doubling time, and had equal efficiency in differentiating to various lineages, when subjected to standard directed differentiation protocols. This paper establishes that discarded High Carries Tooth can be a good source for regenerative medicine and also could be a potential source for MSCs and dental pulp MSC banking.

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来源期刊
Journal of Stem Cells
Journal of Stem Cells Medicine-Transplantation
CiteScore
0.10
自引率
0.00%
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1
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