{"title":"印度出现新世界鹅口疮病毒（Abutilon mosaic virus, A New World Begomovirus）的分子证据。","authors":"P Jyothsna, Q M I Haq, P Jayaprakash, V G Malathi","doi":"10.1007/s13337-013-0139-y","DOIUrl":null,"url":null,"abstract":"<p><p>During an investigation in the year 2010, on the weed reservoir of begomovirus, Abutilon pictum showing bright yellow mosaic symptoms was observed in Udhagamandalam, Tamil Nadu, India. The complete bipartite genome of a begomovirus was cloned and sequenced which revealed association of Abutilon mosaic virus (AbMV). Nicotiana benthamiana plants inoculated biolistically with the concatemers generated through rolling circle amplification of the cloned DNAs were asymptomatic; however three out of nine plants showed presence of viral DNA A. A recombination event in the ORF BC1 with ToLCNDV DNA B (HM989846) was detected. This is the first molecular evidence of AbMV in India. </p>","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 2","pages":"284-8"},"PeriodicalIF":0.0000,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784915/pdf/13337_2013_Article_139.pdf","citationCount":"0","resultStr":"{\"title\":\"Molecular Evidence for the Occurrence of Abutilon mosaic virus, A New World Begomovirus in India.\",\"authors\":\"P Jyothsna, Q M I Haq, P Jayaprakash, V G Malathi\",\"doi\":\"10.1007/s13337-013-0139-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>During an investigation in the year 2010, on the weed reservoir of begomovirus, Abutilon pictum showing bright yellow mosaic symptoms was observed in Udhagamandalam, Tamil Nadu, India. The complete bipartite genome of a begomovirus was cloned and sequenced which revealed association of Abutilon mosaic virus (AbMV). Nicotiana benthamiana plants inoculated biolistically with the concatemers generated through rolling circle amplification of the cloned DNAs were asymptomatic; however three out of nine plants showed presence of viral DNA A. A recombination event in the ORF BC1 with ToLCNDV DNA B (HM989846) was detected. This is the first molecular evidence of AbMV in India. </p>\",\"PeriodicalId\":50370,\"journal\":{\"name\":\"Indian Journal of Virology\",\"volume\":\"24 2\",\"pages\":\"284-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784915/pdf/13337_2013_Article_139.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Virology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s13337-013-0139-y\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2013/6/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Virology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s13337-013-0139-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2013/6/5 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
2010 年，在印度泰米尔纳德邦乌达加曼丹拉姆（Udhagamandalam）进行的一项关于乞猴病毒杂草储库的调查中，观察到 Abutilon pictum 出现了鲜黄色的马赛克症状。克隆并测序了一种begomovirus的完整双组基因组，发现它与Abutilon mosaic virus（AbMV）有关。用通过克隆 DNA 的滚动圈扩增产生的共轭物对烟草植物进行生物接种，发现植物无症状；但 9 株植物中有 3 株显示出病毒 DNA A 的存在。这是印度首次发现 AbMV 的分子证据。
Molecular Evidence for the Occurrence of Abutilon mosaic virus, A New World Begomovirus in India.
During an investigation in the year 2010, on the weed reservoir of begomovirus, Abutilon pictum showing bright yellow mosaic symptoms was observed in Udhagamandalam, Tamil Nadu, India. The complete bipartite genome of a begomovirus was cloned and sequenced which revealed association of Abutilon mosaic virus (AbMV). Nicotiana benthamiana plants inoculated biolistically with the concatemers generated through rolling circle amplification of the cloned DNAs were asymptomatic; however three out of nine plants showed presence of viral DNA A. A recombination event in the ORF BC1 with ToLCNDV DNA B (HM989846) was detected. This is the first molecular evidence of AbMV in India.
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