内源性P物质的恢复与糖尿病大鼠视网膜细胞凋亡的抑制有关

Regulatory Peptides Pub Date : 2013-11-10 DOI:10.1016/j.regpep.2013.09.001

Ji-Hong Yang , Zheng Guo , Ting Zhang , Xian Xian Meng , Li-Sha Xie

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引用次数: 11

摘要

本研究旨在探讨糖尿病大鼠视网膜神经元P物质(SP)的变化及其与凋亡的关系。以链脲佐菌素诱导的糖尿病大鼠为研究对象。采用酶联免疫吸附法和逆转录聚合酶链反应检测SP及其mRNA的变化。研究了辣椒素(20 mg/kg, s.c)恢复SP水平对视网膜细胞凋亡的影响。通过观察视网膜细胞凋亡率和caspase-3活性的变化来评价视网膜细胞凋亡情况。结果发现，与对照组相比，在糖尿病诱导10周时，视网膜细胞凋亡增加了3.5倍，同时视网膜中SP蛋白减少了28%，mRNA减少了32%。辣椒素显著提高糖尿病大鼠视网膜内源性SP mRNA和蛋白含量，分别提高29%和17%，并显著抑制糖尿病大鼠视网膜细胞凋亡和caspase-3活性。糖尿病诱导视网膜细胞凋亡增加，SP降低。辣椒素预处理可逆转糖尿病大鼠视网膜内源性SP的减少和细胞凋亡的增加。视网膜SP的恢复可能是预防糖尿病发展过程中视网膜损伤的一种新的选择。

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Restoration of endogenous substance P is associated with inhibition of apoptosis of retinal cells in diabetic rats

This study was designed to investigate the alterations of substance P (SP) and its correlation with apoptosis of the retinal neurons in diabetic rats.

The study was carried out with diabetic rats induced by streptozotocin. Changes of SP and its mRNA were examined using enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction. The effect of restoration of SP level by capsaicin (20 mg/kg, s.c.) on the apoptosis of the retinal cells was studied. The apoptosis was evaluated by change of ratio of the apoptotic cells and caspase-3 activity in the retina.

It was found that increase in apoptosis of retinal cells, by 3.5 fold of control, was accompanied by reduction of SP, by 28% in protein and 32% in the mRNA in the retina at 10 weeks of induction of diabetes, compared to the controls. Capsaicin significantly elevated endogenous SP, by 29% in the mRNA and 17% in protein in the retina, with marked inhibition of the apoptosis and the activity of caspase-3 in the diabetic rats.

Induction of diabetes leads to the increase of cell apoptosis and the decrease of SP in the retina. The reduction of the endogenous SP and the increase of the cell apoptosis in the retina of the diabetic rats were reversed by pretreatment with capsaicin. Restoration of SP in the retina may be a novel option for prevention of the retinal injury during development of diabetes.

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.