Anna V. Kutina, Anna S. Marina, Elena I. Shakhmatova, Yury V. Natochin
{"title":"血管催产素在大鼠中具有选择性利钠、利尿和抗利尿作用的类似物","authors":"Anna V. Kutina, Anna S. Marina, Elena I. Shakhmatova, Yury V. Natochin","doi":"10.1016/j.regpep.2013.06.013","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>The aim of the present study was an investigation of mechanisms mediating selective effect of vasotocin analogues on water, sodium, and </span>potassium excretion. We tested vasotocin analogues: Mpa</span><sup>1</sup>-vasotocin (dAVT), Mpa<sup>1</sup>-Arg<sup>4</sup>-vasotocin (dAAVT) and Mpa<sup>1</sup>-DArg<sup>8</sup><span><span><span>-vasotocin (dDAVT). The effects on water, sodium, and potassium transport were evaluated in experiments using normal and water-loaded </span>Wistar rats<span>. It was shown that all tested peptides exerted antidiuretic<span> activity. Vasotocin and dAVT induced natriuresis and </span></span></span>kaliuresis in rats. V</span><sub>1a</sub> agonist (Phe<sup>2</sup>-Ile<sup>3</sup>-Orn<sup>8</sup><span>-vasopressin) reproduced the renal effects of dAVT on sodium and potassium excretion but not on water reabsorption. dAAVT, dDAVT and V</span><sub>2</sub><span> agonist (desmopressin) induced kaliuresis without any effect on sodium excretion. Natriuresis was associated with increase in cGMP excretion, whereas kaliuresis was correlated with rise of cAMP excretion. V</span><sub>1a</sub> antagonist (Pmp<sup>1</sup>-Tyr(Me)<sup>2</sup>-vasopressin) significantly reduced the dAVT-stimulated natriuresis and did not influence on urinary potassium excretion. V<sub>2</sub> antagonist (Pmp<sup>1</sup>-DIle<sup>2</sup>-Ile<sup>4</sup><span>-vasopressin) significantly reduced the dAVT- and dAAVT-induced kaliuresis. It is assumed that effects of the nonapeptides on sodium and potassium transport are independent of their antidiuretic activity and mediated by different subtypes of V receptors (the V</span><sub>1a</sub> or V<sub>1a</sub>-like receptor for natriuretic effect and V<sub>2</sub> or V<sub>2</sub><span>-like one for kaliuretic). In accordance to the data obtained, there is a possibility of selective regulation of renal water reabsorption and urinary sodium and potassium excretion with involvement of neurohypophysial hormones.</span></p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2013.06.013","citationCount":"7","resultStr":"{\"title\":\"Vasotocin analogues with selective natriuretic, kaliuretic and antidiuretic effects in rats\",\"authors\":\"Anna V. Kutina, Anna S. Marina, Elena I. Shakhmatova, Yury V. Natochin\",\"doi\":\"10.1016/j.regpep.2013.06.013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>The aim of the present study was an investigation of mechanisms mediating selective effect of vasotocin analogues on water, sodium, and </span>potassium excretion. We tested vasotocin analogues: Mpa</span><sup>1</sup>-vasotocin (dAVT), Mpa<sup>1</sup>-Arg<sup>4</sup>-vasotocin (dAAVT) and Mpa<sup>1</sup>-DArg<sup>8</sup><span><span><span>-vasotocin (dDAVT). The effects on water, sodium, and potassium transport were evaluated in experiments using normal and water-loaded </span>Wistar rats<span>. It was shown that all tested peptides exerted antidiuretic<span> activity. Vasotocin and dAVT induced natriuresis and </span></span></span>kaliuresis in rats. V</span><sub>1a</sub> agonist (Phe<sup>2</sup>-Ile<sup>3</sup>-Orn<sup>8</sup><span>-vasopressin) reproduced the renal effects of dAVT on sodium and potassium excretion but not on water reabsorption. dAAVT, dDAVT and V</span><sub>2</sub><span> agonist (desmopressin) induced kaliuresis without any effect on sodium excretion. Natriuresis was associated with increase in cGMP excretion, whereas kaliuresis was correlated with rise of cAMP excretion. V</span><sub>1a</sub> antagonist (Pmp<sup>1</sup>-Tyr(Me)<sup>2</sup>-vasopressin) significantly reduced the dAVT-stimulated natriuresis and did not influence on urinary potassium excretion. V<sub>2</sub> antagonist (Pmp<sup>1</sup>-DIle<sup>2</sup>-Ile<sup>4</sup><span>-vasopressin) significantly reduced the dAVT- and dAAVT-induced kaliuresis. It is assumed that effects of the nonapeptides on sodium and potassium transport are independent of their antidiuretic activity and mediated by different subtypes of V receptors (the V</span><sub>1a</sub> or V<sub>1a</sub>-like receptor for natriuretic effect and V<sub>2</sub> or V<sub>2</sub><span>-like one for kaliuretic). In accordance to the data obtained, there is a possibility of selective regulation of renal water reabsorption and urinary sodium and potassium excretion with involvement of neurohypophysial hormones.</span></p></div>\",\"PeriodicalId\":20853,\"journal\":{\"name\":\"Regulatory Peptides\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-08-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.regpep.2013.06.013\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Regulatory Peptides\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167011513000992\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regulatory Peptides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167011513000992","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Vasotocin analogues with selective natriuretic, kaliuretic and antidiuretic effects in rats
The aim of the present study was an investigation of mechanisms mediating selective effect of vasotocin analogues on water, sodium, and potassium excretion. We tested vasotocin analogues: Mpa1-vasotocin (dAVT), Mpa1-Arg4-vasotocin (dAAVT) and Mpa1-DArg8-vasotocin (dDAVT). The effects on water, sodium, and potassium transport were evaluated in experiments using normal and water-loaded Wistar rats. It was shown that all tested peptides exerted antidiuretic activity. Vasotocin and dAVT induced natriuresis and kaliuresis in rats. V1a agonist (Phe2-Ile3-Orn8-vasopressin) reproduced the renal effects of dAVT on sodium and potassium excretion but not on water reabsorption. dAAVT, dDAVT and V2 agonist (desmopressin) induced kaliuresis without any effect on sodium excretion. Natriuresis was associated with increase in cGMP excretion, whereas kaliuresis was correlated with rise of cAMP excretion. V1a antagonist (Pmp1-Tyr(Me)2-vasopressin) significantly reduced the dAVT-stimulated natriuresis and did not influence on urinary potassium excretion. V2 antagonist (Pmp1-DIle2-Ile4-vasopressin) significantly reduced the dAVT- and dAAVT-induced kaliuresis. It is assumed that effects of the nonapeptides on sodium and potassium transport are independent of their antidiuretic activity and mediated by different subtypes of V receptors (the V1a or V1a-like receptor for natriuretic effect and V2 or V2-like one for kaliuretic). In accordance to the data obtained, there is a possibility of selective regulation of renal water reabsorption and urinary sodium and potassium excretion with involvement of neurohypophysial hormones.
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Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.
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