Mads Gabrielsen, M Florencia Rey-Burusco, Kate Griffiths, Andrew J Roe, Alan Cooper, Brian O Smith, Malcolm W Kennedy, Betina Corsico
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引用次数: 0
摘要
Na-FAR-1 是一种不寻常的富含 α-helix 的脂肪酸和视黄醇结合蛋白，来自 Necator americanus（一种以血为食的人类肠道寄生线虫）。它属于线虫特有的 FAR 蛋白家族；迄今为止还没有关于寄生虫 FAR 蛋白的结构信息。获得的晶体有两种不同的形态，分别对应不同的空间群。晶体形态 1 显示空间群 P432（单位胞参数 a = b = c = 120.80 Å，α = β = γ = 90°），衍射分辨率为 2.5 Å，而晶体形态 2 显示空间群 F23（单位胞参数 a = b = c = 240.38 Å，α = β = γ = 90°），衍射分辨率为 3.2 Å。晶体形态 2 显示出明显的孪晶迹象。
Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus.
Na-FAR-1 is an unusual α-helix-rich fatty acid- and retinol-binding protein from Necator americanus, a blood-feeding intestinal parasitic nematode of humans. It belongs to the FAR protein family, which is unique to nematodes; no structural information is available to date for FAR proteins from parasites. Crystals were obtained with two different morphologies that corresponded to different space groups. Crystal form 1 exhibited space group P432 (unit-cell parameters a = b = c = 120.80 Å, α = β = γ = 90°) and diffracted to 2.5 Å resolution, whereas crystal form 2 exhibited space group F23 (unit-cell parameters a = b = c = 240.38 Å, α = β = γ = 90°) and diffracted to 3.2 Å resolution. Crystal form 2 showed signs of significant twinning.
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