抗痢疾Holarrhena的体内外抗尿石活性研究。

Urological Research Pub Date : 2012-12-01 Epub Date: 2012-05-24 DOI:10.1007/s00240-012-0483-1
Aslam Khan, Saeed R Khan, Anwar H Gilani
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引用次数: 58

摘要

抗痢疾Holarrhena antidysenterica传统上用于治疗尿石症,因此,其粗提物已被研究可能的抗尿石作用。采用体外和体内两种方法对抗痢疾Holarrhena antidysenterica (Ha.Cr)水甲醇粗提物进行了研究。在体外实验中,哈。Cr对聚集斜率有浓度依赖性(0.25 ~ 4 mg/ml)的抑制作用。在草酸钙亚稳溶液中,使一水草酸钙(COM)晶体变小,转化为脱水草酸钙(COD)晶体。对大鼠肾组织匀浆诱导的2,2-二苯基-1-吡啶肼(DPPH)自由基和脂质过氧化也有浓度依赖性的抗氧化作用。哈哈。Cr (0.3 mg/ml)降低(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies on the in vitro and in vivo antiurolithic activity of Holarrhena antidysenterica.

Holarrhena antidysenterica has a traditional use in the treatment of urolithiasis, therefore, its crude extract has been investigated for possible antiurolithic effect. The crude aqueous-methanolic extract of Holarrhena antidysenterica (Ha.Cr) was studied using the in vitro and in vivo methods. In the in vitro experiments, Ha.Cr demonstrated a concentration-dependent (0.25-4 mg/ml) inhibitory effect on the slope of aggregation. It decreased the size of crystals and transformed the calcium oxalate monohydrate (COM) to calcium oxalate dehydrate (COD) crystals, in calcium oxalate metastable solutions. It also showed concentration-dependent antioxidant effect against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and lipid peroxidation induced in rat kidney tissue homogenate. Ha.Cr (0.3 mg/ml) reduced (p < 0.05) the cell toxicity and LDH release in renal epithelial cells (MDCK) exposed to oxalate (0.5 mM) and COM (66 μg/cm(2)) crystals. In male Wistar rats, receiving 0.75 % ethylene glycol (EG) for 21 days along with 1 % ammonium chloride (AC) in drinking water, Ha.Cr treatment (30-100 mg/kg) prevented the toxic changes caused by lithogenic agents; EG and AC, like loss of body weight, polyurea, oxaluria, raised serum urea and creatinine levels and crystal deposition in kidneys compared to their respective controls. These data indicate that Holarrhena antidysenterica possesses antiurolithic activity, possibly mediated through the inhibition of CaOx crystal aggregation, antioxidant and renal epithelial cell protective activities and may provide base for designing future studies to establish its efficacy and safety for clinical use.

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来源期刊
Urological Research
Urological Research 医学-泌尿学与肾脏学
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