Frank Haubner, Elisabeth Ohmann, Uwe Müller-Vogt, Peter Kummer, Juergen Strutz, Holger G Gassner, Holger Gassner
{"title":"肉毒毒素a对皮肤瘢痕细胞培养模型中细胞因子合成的影响。","authors":"Frank Haubner, Elisabeth Ohmann, Uwe Müller-Vogt, Peter Kummer, Juergen Strutz, Holger G Gassner, Holger Gassner","doi":"10.1001/archfacial.2011.734","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To evaluate possible botulinum toxin A effects in a cell culture model.</p><p><strong>Methods: </strong>In a cell culture model with dermal fibroblasts and microvascular endothelial cells, possible botulinum toxin A effects were evaluated. Cell proliferation and cytokine expression were analyzed using viability assays and enzyme-linked immunosorbent assay techniques.</p><p><strong>Results: </strong>Neither cell proliferation nor cytokines and growth factors (interleukin 6, monocyte chemoattractant protein 2, fibroblast growth factor, macrophage colony-stimulating factor, and vascular endothelial growth factor) were affected by botulinum toxin A incubation.</p><p><strong>Conclusions: </strong>The present data do not add evidence to suggest a significant therapeutic role of botulinum toxin A injections for cutaneous wound healing beyond chemoimmobilization. Further studies that include patient-specific cells of hypertrophic scars are required to better understand what role botulinum toxin A can play in the treatment of mature scar tissue.</p>","PeriodicalId":55470,"journal":{"name":"Archives of Facial Plastic Surgery","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1001/archfacial.2011.734","citationCount":"22","resultStr":"{\"title\":\"Effects of botulinum toxin a on cytokine synthesis in a cell culture model of cutaneous scarring.\",\"authors\":\"Frank Haubner, Elisabeth Ohmann, Uwe Müller-Vogt, Peter Kummer, Juergen Strutz, Holger G Gassner, Holger Gassner\",\"doi\":\"10.1001/archfacial.2011.734\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To evaluate possible botulinum toxin A effects in a cell culture model.</p><p><strong>Methods: </strong>In a cell culture model with dermal fibroblasts and microvascular endothelial cells, possible botulinum toxin A effects were evaluated. Cell proliferation and cytokine expression were analyzed using viability assays and enzyme-linked immunosorbent assay techniques.</p><p><strong>Results: </strong>Neither cell proliferation nor cytokines and growth factors (interleukin 6, monocyte chemoattractant protein 2, fibroblast growth factor, macrophage colony-stimulating factor, and vascular endothelial growth factor) were affected by botulinum toxin A incubation.</p><p><strong>Conclusions: </strong>The present data do not add evidence to suggest a significant therapeutic role of botulinum toxin A injections for cutaneous wound healing beyond chemoimmobilization. Further studies that include patient-specific cells of hypertrophic scars are required to better understand what role botulinum toxin A can play in the treatment of mature scar tissue.</p>\",\"PeriodicalId\":55470,\"journal\":{\"name\":\"Archives of Facial Plastic Surgery\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1001/archfacial.2011.734\",\"citationCount\":\"22\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Facial Plastic Surgery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1001/archfacial.2011.734\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2011/10/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Facial Plastic Surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1001/archfacial.2011.734","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2011/10/17 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of botulinum toxin a on cytokine synthesis in a cell culture model of cutaneous scarring.
Objective: To evaluate possible botulinum toxin A effects in a cell culture model.
Methods: In a cell culture model with dermal fibroblasts and microvascular endothelial cells, possible botulinum toxin A effects were evaluated. Cell proliferation and cytokine expression were analyzed using viability assays and enzyme-linked immunosorbent assay techniques.
Results: Neither cell proliferation nor cytokines and growth factors (interleukin 6, monocyte chemoattractant protein 2, fibroblast growth factor, macrophage colony-stimulating factor, and vascular endothelial growth factor) were affected by botulinum toxin A incubation.
Conclusions: The present data do not add evidence to suggest a significant therapeutic role of botulinum toxin A injections for cutaneous wound healing beyond chemoimmobilization. Further studies that include patient-specific cells of hypertrophic scars are required to better understand what role botulinum toxin A can play in the treatment of mature scar tissue.
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