M. L. Lázaro-Suárez, J. H. Gómez-Zamudio, N. L. Delgado-Buenrostro, A. Tanoue, G. Tsujimoto, R. Villalobos-Molina
{"title":"血管紧张素II改变α 1d -肾上腺素受体敲除小鼠主动脉平滑肌细胞中α1-肾上腺素受体的表达","authors":"M. L. Lázaro-Suárez, J. H. Gómez-Zamudio, N. L. Delgado-Buenrostro, A. Tanoue, G. Tsujimoto, R. Villalobos-Molina","doi":"10.1111/j.1474-8673.2011.00467.x","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><b>1</b> The effect of angiotensin II (Ang II) on α<sub>1A</sub>-, α<sub>1B</sub>-, and a<sub>1D</sub>-adrenoceptors (α<sub>1</sub>-AR) expression was analyzed in aorta smooth muscle cells obtained from wild-type (WT) and knock out of α<sub>1D</sub>-AR (α<sub>1D</sub>-AR KO) mice.</p>\n <p><b>2</b> The relative abundance of mRNA for the three α<sub>1</sub>-ARs was determined in WT and α<sub>1D</sub>-AR KO aortic smooth muscle cells. There were no significant differences between WT and α<sub>1D</sub>-AR KO cells.</p>\n <p><b>3</b> As early as 1 h Ang II increased α<sub>1B</sub>-AR mRNA in WT cells ≈ 2 fold compared with control; in contrast, in α<sub>1D</sub>-AR KO cells the α<sub>1B</sub>-AR transcript was ≈ 50% of control.</p>\n <p><b>4</b> Western blot assays showed that Ang II incremented protein content for α<sub>1A</sub>-AR, 86% and 107% in WT and α<sub>1D</sub>-AR KO cells, respectively.</p>\n <p><b>5</b> Protein for α<sub>1B</sub>- and α<sub>1D</sub>-ARs did not change significantly with Ang II in both WT and a<sub>1D</sub>-AR KO cells.</p>\n <p><b>6</b> The effect of Ang II on α<sub>1B</sub>-AR mRNA seems to be influenced by the absence of α<sub>1D</sub>-AR in aortic smooth muscle cells, which might be important to understand the interactions among α<sub>1</sub>-ARs.</p>\n </div>","PeriodicalId":100151,"journal":{"name":"Autonomic and Autacoid Pharmacology","volume":"31 3-4","pages":"57-63"},"PeriodicalIF":0.0000,"publicationDate":"2011-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1474-8673.2011.00467.x","citationCount":"2","resultStr":"{\"title\":\"Angiotensin II modifies the expression of α1-adrenoceptors in aorta smooth muscle cells of α1D-adrenoceptor knockout mice\",\"authors\":\"M. L. Lázaro-Suárez, J. H. Gómez-Zamudio, N. L. Delgado-Buenrostro, A. Tanoue, G. Tsujimoto, R. Villalobos-Molina\",\"doi\":\"10.1111/j.1474-8673.2011.00467.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p><b>1</b> The effect of angiotensin II (Ang II) on α<sub>1A</sub>-, α<sub>1B</sub>-, and a<sub>1D</sub>-adrenoceptors (α<sub>1</sub>-AR) expression was analyzed in aorta smooth muscle cells obtained from wild-type (WT) and knock out of α<sub>1D</sub>-AR (α<sub>1D</sub>-AR KO) mice.</p>\\n <p><b>2</b> The relative abundance of mRNA for the three α<sub>1</sub>-ARs was determined in WT and α<sub>1D</sub>-AR KO aortic smooth muscle cells. There were no significant differences between WT and α<sub>1D</sub>-AR KO cells.</p>\\n <p><b>3</b> As early as 1 h Ang II increased α<sub>1B</sub>-AR mRNA in WT cells ≈ 2 fold compared with control; in contrast, in α<sub>1D</sub>-AR KO cells the α<sub>1B</sub>-AR transcript was ≈ 50% of control.</p>\\n <p><b>4</b> Western blot assays showed that Ang II incremented protein content for α<sub>1A</sub>-AR, 86% and 107% in WT and α<sub>1D</sub>-AR KO cells, respectively.</p>\\n <p><b>5</b> Protein for α<sub>1B</sub>- and α<sub>1D</sub>-ARs did not change significantly with Ang II in both WT and a<sub>1D</sub>-AR KO cells.</p>\\n <p><b>6</b> The effect of Ang II on α<sub>1B</sub>-AR mRNA seems to be influenced by the absence of α<sub>1D</sub>-AR in aortic smooth muscle cells, which might be important to understand the interactions among α<sub>1</sub>-ARs.</p>\\n </div>\",\"PeriodicalId\":100151,\"journal\":{\"name\":\"Autonomic and Autacoid Pharmacology\",\"volume\":\"31 3-4\",\"pages\":\"57-63\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1474-8673.2011.00467.x\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Autonomic and Autacoid Pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1474-8673.2011.00467.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Autonomic and Autacoid Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1474-8673.2011.00467.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Angiotensin II modifies the expression of α1-adrenoceptors in aorta smooth muscle cells of α1D-adrenoceptor knockout mice
1 The effect of angiotensin II (Ang II) on α1A-, α1B-, and a1D-adrenoceptors (α1-AR) expression was analyzed in aorta smooth muscle cells obtained from wild-type (WT) and knock out of α1D-AR (α1D-AR KO) mice.
2 The relative abundance of mRNA for the three α1-ARs was determined in WT and α1D-AR KO aortic smooth muscle cells. There were no significant differences between WT and α1D-AR KO cells.
3 As early as 1 h Ang II increased α1B-AR mRNA in WT cells ≈ 2 fold compared with control; in contrast, in α1D-AR KO cells the α1B-AR transcript was ≈ 50% of control.
4 Western blot assays showed that Ang II incremented protein content for α1A-AR, 86% and 107% in WT and α1D-AR KO cells, respectively.
5 Protein for α1B- and α1D-ARs did not change significantly with Ang II in both WT and a1D-AR KO cells.
6 The effect of Ang II on α1B-AR mRNA seems to be influenced by the absence of α1D-AR in aortic smooth muscle cells, which might be important to understand the interactions among α1-ARs.
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