多酚氧化酶包封法测定苯酚的聚吡咯-聚乙烯磺酸盐复合膜生物传感器的制备。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2011-10-01 DOI:10.3109/10731199.2011.585616

Halit Arslan, Fatma Arslan

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引用次数: 7

摘要

摘要:本文通过铂电极表面的包埋技术，将多酚氧化酶(酪氨酸酶)固定在电化学聚合聚吡咯-聚乙烯磺酸钠(py - pvs)薄膜上，实现了一种新型的电流型苯酚生物传感器。安培法的测定是基于苯酚酶促反应中醌的电化学还原。考察了pH和温度的影响，确定最佳工艺条件为8.0℃和30℃。在1.0 × 10(-7) ~ 5.0 × 10(-6) m的线性工作范围内，研究了酶电极的贮存稳定性和操作稳定性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Preparation of a polypyrrole-polyvinylsulphonate composite film biosensor for determination of phenol based on entrapment of polyphenol oxidase.

Abstract: In this paper, a novel amperometric phenol biosensor with immobilization of polyphenol oxidase (tyrosinase) on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique on the surface of a platinum electrode. The amperometric determination is based on the electrochemical reduction of quinon generated in the enzymatic reaction of phenol. The effects of pH and temperature were investigated and optimum parameters were found to be 8.0 and 30 °C, respectively. The linear working range of the electrode was 1.0 × 10(-7) - 5.0 × 10(-6) M. The storage stability and operation stability of the enzyme electrode were also studied.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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6-12 weeks