Pamela D McLaughlin, Wayne A McLaughlin, Douglas P Maxwell, Marcia E Roye
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引用次数: 0
摘要
在三年的时间里,我们从伯利兹的五个地区收集了包括辣椒、红芸豆、南瓜、四季豆和西红柿在内的植物以及带有病毒症状的杂草,目的是确定乞猴病毒的多样性。通过 DNA 杂交筛选出的样本中,有 65% 产生了乞蛾病毒感染信号。随后的 PCR 扩增和核苷酸序列分析表明,伯利兹存在四种乞猴病毒。发现辣椒金色镶嵌病毒和番茄斑驳病毒-[Flo]与番茄和甜椒有关,前者还从辣椒中分离出来。在辣椒、甜椒和杂草物种 Euphorbia heterophylla 上发现了 Merremia 镶嵌病毒。在辣椒和大戟科植物中发现了大戟科花叶病毒-[尤卡坦半岛]。这是伯利兹首次发现乞猴病毒。
Identification of Begomoviruses Infecting Crops and Weeds in Belize.
Plants including pepper, red kidney bean, squash, string bean and tomato, as well as weeds with viral symptoms were collected from five districts in Belize over a three year period with the aim of determining the diversity of the begomoviruses present. Sixty five percent of the samples screened via DNA hybridization produced signals indicative of begomovirus infection. Subsequent PCR amplifications and nucleotide sequence analyses revealed the presence of four begomoviruses in Belize. Pepper golden mosaic virus and Tomato mottle virus-[Flo] were found associated with tomato and sweet pepper and the former was also isolated from hot pepper. Merremia mosaic virus was found infecting hot pepper, sweet pepper and the weed species Euphorbia heterophylla. Euphorbia mosaic virus-[Yucatan Peninsula] was found in hot pepper and Euphorbia. This is the first report of the identification of begomoviruses in Belize.