[一步逆转录-聚合酶链反应法检测腮腺炎病毒疏水酶基因的建立]。

中国疫苗和免疫 Pub Date : 2010-02-01

Xin Chang, Jian-Hui Zhou, Shuang Wang

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引用次数: 0

摘要

目的:建立一种简便的腮腺炎病毒基因鉴定方法:一步逆转录聚合酶链反应(RT-PCR)。方法:在SH基因两侧设计引物，进行RT-PCR法的敏感性和特异性试验。结果:该方法至少可检出10株(0.7)TCID50腮腺炎病毒。该方法可检出5株腮腺炎病毒，不能检出4株非腮腺炎病毒，包括麻疹病毒和风疹病毒。结论:一步RT-PCR法是一种快速、简便的腮腺炎病毒基因鉴定方法。

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[Establishment of one-step reverse transcription-polymerase chain reaction method for mumps virus hydrophobin gene detection].

Objective: To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).

Methods: To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.

Results: At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.

Conclusions: The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.

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中国疫苗和免疫

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