rDNA基因的物理定位证实了无融合Brachiaria brizantha异源多倍体的起源。

Sexual Plant Reproduction Pub Date : 2010-03-01 Epub Date: 2009-12-15 DOI:10.1007/s00497-009-0124-1
Stephan Nielen, Lucas M Almeida, Vera T C Carneiro, Ana Claudia G Araujo
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引用次数: 12

摘要

Brachiaria(指标)。Griseb属于Poaceae家族,在属内,在同一物种的不同品种中存在无融合或性行为。腕鱼属植物大多数是多倍体和无生殖体,这使得通过育种来改良作物的策略非常复杂。尽管无融合性多倍体的频率很高,但在腕鱼中,多倍体和杂交与无融合性的关系尚不清楚。进一步的分析需要关于多倍体基因组组成的详细知识。本文介绍了荧光原位杂交(FISH)技术在腕鱼细胞遗传学分析中的应用。对brizantha二倍体有性系(BRA 002747)和四倍体无染色体系(BRA000591)的常规核型进行物理定位,证明后者是异源多倍体起源。根据我们的研究结果和先前对棘球菊无融合的认识,我们认为无融合的起源可能是杂交的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physical mapping of rDNA genes corroborates allopolyploid origin in apomictic Brachiaria brizantha.

Brachiaria (Trin.) Griseb belongs to the family Poaceae, and within the genus, apomixis or sexuality is present in different accessions of the same species. The majority of Brachiaria species are polyploid and apomictic, making strategies for crop improvement by breeding very intricate. In spite of the high frequency of apomictic polyploids, the relationship of polyploidy and hybridization with apomixis in Brachiaria is still unclear. Further analysis requires detailed knowledge regarding the genomic composition of the polyploids. The present work introduces the use of fluorescent in situ hybridization (FISH) into cytogenetic analysis of Brachiaria. Physical mapping of heterologous rDNA sequences, associated with conventional karyotyping of the B. brizantha diploid sexual (BRA 002747) and the tetraploid apomictic (BRA000591) accessions, provided evidence of the latter being of allopolyploid origin. Based on our results and on previous knowledge on apomixis in B. brizantha, we suggest that the origin of apomixis was probably a consequence of hybridization.

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来源期刊
Sexual Plant Reproduction
Sexual Plant Reproduction 生物-生殖生物学
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