用聚合酶反应评价2'-脱氧核苷n -氧化物衍生物的碱基识别能力。

Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine
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引用次数: 0

摘要

双氧水氧化胞嘧啶和腺嘌呤碱得到的主要产物是胞嘧啶和腺嘌呤n -氧化物衍生物。这些n -氧化物衍生物有可能像8-氧鸟嘌呤或胸腺嘧啶乙二醇一样在基因组DNA中具有诱变作用。虽然2'-脱氧核苷n -氧化物衍生物的化学合成和性质已经得到了很好的证实,但关于含有这些修饰的2'-脱氧核苷的寡脱氧核苷酸(ODNs)的化学和生化行为的报道很少。在本研究中,我们通过DNA聚合酶反应检测了它们的碱基识别能力。研究发现,这些修饰的衍生物通过DNA聚合酶精确地选择模板ODN上互补的G或T碱基,被整合到ODN的3'端。在含有2'-脱氧核苷n -氧化物碱基的模板odn掺入反应中,它们的n -氧化物碱基被互补的5'-三磷酸(dGTP或dTTP)选择性识别。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of base recognition abilities of 2'-deoxynucleoside N-oxide derivatives by polymerase reactions.
The main products obtained by oxidation of cytosine and adenine bases with hydrogen peroxide are cytosine and adenine N-oxide derivatives. There is a possibility that these N-oxide derivatives are mutagenic in genomic DNA like 8-oxoguanine or thymine glycol. Although the chemical synthesis and properties of 2'-deoxynucleoside N-oxide derivatives have been well established, little has been reported about the chemical and biochemical behavior of oligodeoxynucleotides (ODNs) containing these modified 2'-deoxynucleoside. In this study, we examined their base recognition ability by DNA polymerase reactions. It was found that these modified derivatives were incorporated into the 3'-terminal site of an ODN by DNA polymerase selecting accurately the complementary G or T base on a template ODN. In the incorporation reaction using template ODNs containing 2'-deoxynucleoside N-oxide bases, their N-oxide bases were selectively recognized by the complementary 5'-triphosphate (dGTP or dTTP).
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