功能转移ODN探针对RNA的位点特异性修饰。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp034

Kazumitsu Onizuka, Yosuke Taniguchi, Takamasa Nishioka, Shigeki Sasaki

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引用次数: 3

摘要

RNA分子修饰的有效方法已被期望作为创新的生物工具和治疗方法。在这项研究中，开发了一种通过功能转移ODN探针进行位点特异性RNA修饰的通用方法。通过功能转移反应实现了位点特异性和胞嘧啶选择性RNA修饰。结果表明，碱基选择性和位点选择性是由于DNA-RNA双链中反应物的接近性所致。

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Site-specific modification of RNA by functionality-transfer ODN probes.

Efficient methods for the modification of RNA molecules have been expected as innovative biological tools and therapeutic methods. In this study, the development of a general method for site-specific RNA modification by the functionality-transfer ODN probes has been investigated. Site-specific and cytosine-selective RNA modifications were achieved by the functionality-transfer reaction. It was shown that the base and site-selectivity were due to the close proximity of the reactants in the DNA-RNA duplexes.

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Nucleic acids symposium series (2004)

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