利用细胞膜亲和层析技术研究生物系统中的分子识别。

Q4 Chemistry

Chimica Oggi-chemistry Today Pub Date : 2008-09-01

Irving W Wainer

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引用次数: 0

摘要

细胞膜亲和层析（CMAC）柱是通过将细胞膜片段固定在液相层析支持物上而制成的。含有人类有机阳离子转运体的 CMAC 柱（CMAC(hOCT1) 柱）被用于研究竞争性抑制剂的立体选择性结合。利用 CMAC(hOCT1) 柱获得的色谱数据建立了一个描述立体选择性的药效模型。结果表明，基于抑制剂和 hOCT1 之间构象调整的动态手性识别模型是观察到的立体选择性的原因。

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Investigation of molecular recognition in biological systems using cellular membrane affinity chromatography.

Cellular membrane affinity chromatography (CMAC) columns have been created through the immobilization of cellular membrane fragments on liquid chromatographic supports. A CMAC column containing the human organic cation transporter, CMAC(hOCT1) column, has been used to study the stereoselective binding of competitive inhibitors. The chromatographic data obtained using the CMAC(hOCT1) column was to develop a pharmacophore model that described the stereoselectivity. The results indicate that a dynamic chiral recognition model based upon conformational adjustments between the inhibitors and hOCT1 is responsible for the observed steroeselectivity.

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来源期刊

Chimica Oggi-chemistry Today BIOTECHNOLOGY & APPLIED MICROBIOLOGY-CHEMISTRY, MULTIDISCIPLINARY

CiteScore

0.20

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Chimica Oggi – Chemistry Today is a peer reviewed, bimonthly journal, of the TKS TeknoScienze Publisher. It deals with Fine Chemicals, Applied Chemistry and Biotechnology. Founded in 1983 Chimica Oggi – Chemistry Today soon became a leading journal in linking industry and academia and gained an immediate appreciation worldwide.