Shi Feng Hu, Mu Lan Zhu, Li Xing Liu, Jun Tang, Bi Da Gao, Jie Chen
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引用次数: 0
摘要
为了阐明产生纤维素酶的木霉突变体的表型变化，采用2-DE法检测了koningii T. CICC及其突变体TK-2R-1的蛋白谱。通过MS-TOF-TOF鉴定出8个独特位点，其中4个位点的功能与木霉的生长和代谢有关，为构建木霉生产高效纤维素酶的突变体提供了新的理论依据和方法。
[Analysis of differential protein expression of REMI mutant of Trichoderma koningii].
On purpose of expounding the phenotype change of Trichoderma mutant which produces cellulase, protein profiles of T. koningii CICC and its mutant TK-2R-1 were detected by 2-DE. Eight unique spots were found and four of them were identified and their functions by MS-TOF-TOF were connected with growth and metabolization of Trichoderma spp. The research provides a new theoretical basis and methods for the construction of Trichoderma mutant which is used to produce efficient cellulase.
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