豚鼠味蕾中存在谷胱甘肽免疫反应细胞亚型。

Q4 Medicine

Archives of histology and cytology Pub Date : 2007-12-01 DOI:10.1679/aohc.70.291

Yasuhiro Ohkubo, Hiroyuki Yokosuka, Masahiko Kumakura, Sumio Yoshie

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引用次数: 5

摘要

用免疫组织化学方法研究了豚鼠舌腭味蕾中gustducin与calbinin - d28k (=spot 35蛋白)和III型肌醇三磷酸受体(IP(3)R-3)共定位的情况，以表征gustducin免疫反应细胞。个体味蕾细胞对这三种分子的免疫反应从完全阳性到完全阴性不等。在免疫反应细胞中，gustducin免疫反应细胞分为两个细胞群:一个对calbinin - d28k免疫阳性，另一个免疫阴性。将我们以前的数据与本研究的结果相结合，前一种细胞应该属于电子显微镜指定的III型细胞。这一发现为豚鼠味蕾中表达gustducin的细胞类型提供了新的证据。

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Existence of subtypes of gustducin-immunoreactive cells in the vallate taste bud of guinea pigs.

Vallate taste buds in the guinea-pig tongue were immunohistochemically investigated with regard to the colocalization of gustducin with calbindin-D28K (=spot 35 protein) and type III inositol triphosphate receptor (IP(3)R-3) in order to characterize gustducin-immunoreactive cells. Individual taste bud cells ranged from totally immunopositive to totally immunonegative for these three molecules. Among the immunoreactive cells, gustducin-immunoreactive cells were divided into two cell populations: one immunopositive and the other immunonegative for calbindin-D28K. Applying our previous data to the present results, the former cells should belong to Type III cells designated by electron microscopy. This finding provides new evidence regarding the taste bud types of cells expressing gustducin in the guinea pig.

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来源期刊

Archives of histology and cytology 生物-细胞生物学

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期刊介绍： The Archives of Histology and Cytology provides prompt publication in English of original works on the histology and histochemistry of man and animals. The articles published are in principle restricted to studies on vertebrates, but investigations using invertebrates may be accepted when the intention and results present issues of common interest to vertebrate researchers. Pathological studies may also be accepted, if the observations and interpretations are deemed to contribute toward increasing knowledge of the normal features of the cells or tissues concerned. This journal will also publish reviews offering evaluations and critical interpretations of recent studies and theories.