D Römermann, B Hasemeier, K Metzig, B Schlegelberger, F Länger, H Kreipe, U Lehmann
{"title":"[LINE-1序列在MDS或继发性AML患者中的甲基化状态]。","authors":"D Römermann, B Hasemeier, K Metzig, B Schlegelberger, F Länger, H Kreipe, U Lehmann","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>This study analyzes changes in the degree of global methylationlevel in myelodysplastic syndrome during progression of the disease.</p><p><strong>Methods: </strong>Methylation status was analyzed in 127 patients with histologically confirmed MDS and 26 reactive controls. We employed Pyrosequencing, Luminometric Methylation Assay (LUMA) and a realtime PCR-based quantitative assay.</p><p><strong>Results: </strong>We detected an increase of methylation level of LINE-1 sequences using pyrosequencing and an increase of methylation in the HpaII recognition site employing LUMA during the progression of MDS. Methylation sensitive quantitative PCR showed no statistically significant differences, only a trend.</p><p><strong>Conclusions: </strong>LINE-1 and methylation sensitive cleavage of DNA can act as a surrogatmarker for global DNA methylation. The genome wide hypermethylation of MDS is a distinct feature of this disease. It discriminates MDS from other neoplasia and may explains the success of hypomethylation inducing reagents like azadeoxycytidine in MDS therapy.</p>","PeriodicalId":76792,"journal":{"name":"Verhandlungen der Deutschen Gesellschaft fur Pathologie","volume":"91 ","pages":"338-42"},"PeriodicalIF":0.0000,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Methylation status of LINE-1 sequences in patients with MDS or secondary AML].\",\"authors\":\"D Römermann, B Hasemeier, K Metzig, B Schlegelberger, F Länger, H Kreipe, U Lehmann\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>This study analyzes changes in the degree of global methylationlevel in myelodysplastic syndrome during progression of the disease.</p><p><strong>Methods: </strong>Methylation status was analyzed in 127 patients with histologically confirmed MDS and 26 reactive controls. We employed Pyrosequencing, Luminometric Methylation Assay (LUMA) and a realtime PCR-based quantitative assay.</p><p><strong>Results: </strong>We detected an increase of methylation level of LINE-1 sequences using pyrosequencing and an increase of methylation in the HpaII recognition site employing LUMA during the progression of MDS. Methylation sensitive quantitative PCR showed no statistically significant differences, only a trend.</p><p><strong>Conclusions: </strong>LINE-1 and methylation sensitive cleavage of DNA can act as a surrogatmarker for global DNA methylation. The genome wide hypermethylation of MDS is a distinct feature of this disease. It discriminates MDS from other neoplasia and may explains the success of hypomethylation inducing reagents like azadeoxycytidine in MDS therapy.</p>\",\"PeriodicalId\":76792,\"journal\":{\"name\":\"Verhandlungen der Deutschen Gesellschaft fur Pathologie\",\"volume\":\"91 \",\"pages\":\"338-42\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Verhandlungen der Deutschen Gesellschaft fur Pathologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Verhandlungen der Deutschen Gesellschaft fur Pathologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Methylation status of LINE-1 sequences in patients with MDS or secondary AML].
Objectives: This study analyzes changes in the degree of global methylationlevel in myelodysplastic syndrome during progression of the disease.
Methods: Methylation status was analyzed in 127 patients with histologically confirmed MDS and 26 reactive controls. We employed Pyrosequencing, Luminometric Methylation Assay (LUMA) and a realtime PCR-based quantitative assay.
Results: We detected an increase of methylation level of LINE-1 sequences using pyrosequencing and an increase of methylation in the HpaII recognition site employing LUMA during the progression of MDS. Methylation sensitive quantitative PCR showed no statistically significant differences, only a trend.
Conclusions: LINE-1 and methylation sensitive cleavage of DNA can act as a surrogatmarker for global DNA methylation. The genome wide hypermethylation of MDS is a distinct feature of this disease. It discriminates MDS from other neoplasia and may explains the success of hypomethylation inducing reagents like azadeoxycytidine in MDS therapy.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
