利用间充质干细胞和磷灰石-硅灰石玻璃陶瓷开发定制骨支架。

Jennifer A Dyson, Paul G Genever, Kenneth W Dalgarno, David J Wood
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引用次数: 74

摘要

临床需要一种新型的骨替代材料,它既能延长种植体的使用寿命,又能完全整合并具有适当的力学性能。我们使用人类间充质干细胞(MSCs)填充多孔磷灰石-硅灰石(A-W)玻璃陶瓷支架,通过层制造技术,选择性激光烧结,来制造定制的骨替代物。使用共聚焦显微镜和扫描电子显微镜确定最佳播种密度,并证明MSCs在培养21天后粘附在a - w支架表面并保持活力。我们发现在支架上生长的MSCs数量在7天内显著增加。通过溴脱氧尿苷掺入,我们证明了MSCs在支架上增殖。利用实时荧光定量PCR技术,我们分析了在不添加成骨补充剂的情况下培养的MSCs中成骨标志物碱性磷酸酶、ⅰ型胶原、Cbfa-1、骨钙素、骨连接素和骨桥蛋白的表达。MSCs在A-W支架上对成骨标志物的表达与在组织培养塑料上相当或显著高于组织培养塑料。我们还发现,与商用磷酸钙支架相比,a - w支架上的碱性磷酸酶活性明显更高。这些结果首次表明了A-W支架的生物相容性和骨支持能力,以及它们作为患者特异性骨替代材料的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of custom-built bone scaffolds using mesenchymal stem cells and apatite-wollastonite glass-ceramics.

There is a clinical need for new bone replacement materials that combine long implant life with complete integration and appropriate mechanical properties. We have used human mesenchymal stem cells (MSCs) to populate porous apatite-wollastonite (A-W) glass-ceramic scaffolds produced by the layer manufacturing technique, selective laser sintering, to create custom-built bone replacements. Confocal and scanning electron microscopy were used to determine optimal seeding densities and to demonstrate that MSCs adhered and retained viability on the surface of A-W scaffolds over a culture period of 21 days. We found a significant increase in the number of MSCs growing on the scaffolds over 7 days. Using bromodeoxyuridine incorporation we demonstrated that MSCs proliferated on the scaffolds. Using real-time PCR we analyzed the expression of the osteogenic markers alkaline phosphatase, collagen type-I, Cbfa-1, osteocalcin, osteonectin, and osteopontin by MSCs cultured in the absence of osteogenic supplements. The expression of the osteogenic markers by MSCs was equivalent to or significantly greater on A-W scaffolds than on tissue culture plastic. We also identified significantly higher alkaline phosphatase activity on A-W compared to a commercial calcium phosphate scaffold. These results indicate for the first time the biocompatibility and osteo-supportive capacity of A-W scaffolds and their potential as patient-specific bone replacement materials.

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Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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