Grady Hanrahan, Florence Tse, Froseen T Dahdouh, Keith Clarke, Frank A Gomez
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Design and development of a flow injection-capillary electrophoresis analyzer employing fiber optic detection.
The development and experimental optimization of a novel flow injection-capillary electrophoresis (FI-CE) analyzer employing UV-visible fiber optic detection is described. The analyzer incorporates a miniature charge-coupled device (CCD) spectrometer and operates in a graphical programming environment. Data from experimental optimization studies and small molecule separations involving affinity capillary electrophoresis (ACE) and indirect detection of anions are presented. Future directions in terms of instrument automation and incorporation into a microfluidic format are also discussed.
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