水稻SA和JA诱导蛋白激酶基因OsSJMK1的分离与鉴定

NING Jing, YUAN Bin, XIE Ka-Bin, HU Hong-Hong, WU Cong-Qing, XIONG Li-Zhong
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引用次数: 22

摘要

利用ja诱导的EST,从水稻(Oryza sativa L.)中分离到一个推定的蛋白激酶基因OsSJMK1的全长cDNA。该基因的预测蛋白由498个氨基酸组成,预测PI值为8.43,包含一个具有双磷酸化激活基序TDY (Thr-Asp-Tyr)的丝裂原活化蛋白激酶(MAPK)结构域和一个具有典型MAPK亚群的c端结构域,OsSJMK1在n端激酶结构域与报道的BWMK1(一种爆炸真菌和损伤诱导蛋白)序列同源性为81%,但在c端结构域的序列同源性较低。尽管OsSJMK1与BWMK1基因结构高度相似,但OsSJMK1的表达在处理初期受到茉莉酸(jasmonic acid, JA)和水杨酸(salicylic acid, SA)的短暂诱导,而不受爆炸菌的弱诱导。该基因不受其他激素(如ABA)或非生物胁迫(如干旱和盐度)的诱导。该基因在水稻各组织中均有低水平转录。提示OsSJMK1可能被JA和SA特异性激活,参与防御信号通路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and Identification of SA and JA Inducible Protein Kinase Gene OsSJMK1 in Rice

On the basis of a JA-inducible EST, the full length cDNA of a putative protein kinase gene, OsSJMK1, was isolated from rice (Oryza sativa L.). The predicted protein of this gene, consisting of 498 amino acids with a predicted PI value of 8.43, contains a mitogen-activated protein kinase (MAPK) domain with a dual-phosphorylation activation motif TDY (Thr-Asp-Tyr) and a C-terminus domain that feature a typical MAPK belonging to subgroup V, OsSJMK1 showed 81% sequence identity to the reported BWMK1, a blast fungus and wounding inducible protein, in the N-terminus kinase domain but had low sequence identity in the C-terminus domain. Despite the high similarity of gene structure between OsSJMK1 and BWMK1, the expression of OsSJMK1 was weakly induced by blast fungus but not wounding, rather it was transiently induced by jasmonic acid (JA) and salicylic acid (SA) at early stages of treatment. This gene was not induced by other hormones (such as ABA) or abiotic stresses, such as drought and salinity. The low level of transcript of this gene was detected in the various tissues of rice. The results suggest that OsSJMK1 might be activated specifically by JA and SA and involved in defense signaling pathways.

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