{"title":"一种新的原始生殖细胞培养方法。","authors":"Xin Lu, Qing Xu, Xiao Lin Shi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>To investigate the new method that the primordial germ cells (PGCs) can long time propagate, grow and keep potency of differentiation in vitro. PGCs were co-cultured with Sertoli cells (SCs) or with homologue fibroblasts from genital ridge. When co-cultured with SCs, PGCs colonies and times of transfer of culture were greater than those co-cultured with homologue fibroblasts. At present PGCs of co-cultured with SCs have succeeded in subculturing to the 51 generation. SCs can availably enhance propagating ability as well as a long period of time maintain the characteristic of PGCs.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 6","pages":"474-80"},"PeriodicalIF":0.0000,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[A new culture method for primordial germ cell].\",\"authors\":\"Xin Lu, Qing Xu, Xiao Lin Shi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>To investigate the new method that the primordial germ cells (PGCs) can long time propagate, grow and keep potency of differentiation in vitro. PGCs were co-cultured with Sertoli cells (SCs) or with homologue fibroblasts from genital ridge. When co-cultured with SCs, PGCs colonies and times of transfer of culture were greater than those co-cultured with homologue fibroblasts. At present PGCs of co-cultured with SCs have succeeded in subculturing to the 51 generation. SCs can availably enhance propagating ability as well as a long period of time maintain the characteristic of PGCs.</p>\",\"PeriodicalId\":77395,\"journal\":{\"name\":\"Shi yan sheng wu xue bao\",\"volume\":\"38 6\",\"pages\":\"474-80\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Shi yan sheng wu xue bao\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Shi yan sheng wu xue bao","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
To investigate the new method that the primordial germ cells (PGCs) can long time propagate, grow and keep potency of differentiation in vitro. PGCs were co-cultured with Sertoli cells (SCs) or with homologue fibroblasts from genital ridge. When co-cultured with SCs, PGCs colonies and times of transfer of culture were greater than those co-cultured with homologue fibroblasts. At present PGCs of co-cultured with SCs have succeeded in subculturing to the 51 generation. SCs can availably enhance propagating ability as well as a long period of time maintain the characteristic of PGCs.
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