在中国幽门螺杆菌中,消化性溃疡与Lewis抗原表达增加有关,而与空泡细胞毒素活性或babA2基因状态无关。

Peng Yuan Zheng, Fu Ai Tang, Yuan Ming Qi, Jian Li
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引用次数: 7

摘要

目的:幽门螺杆菌西方株和亚洲株的毒力因子vacA、babA2和Lewis血型抗原存在争议。本研究的目的是确定这些潜在毒力因素在中国人群中的重要性。方法:对郑州地区患者分离的72株幽门螺杆菌进行检测,其中消化性溃疡43例,慢性胃炎29例。用HeLa细胞进行空泡细胞毒素测定。采用酶联免疫吸附试验(ELISA)检测Le血型抗原(Le(a)、Le(b)、Le(x)、Le(y))的表达。聚合酶链反应鉴定babA2基因。使用双尾Fisher精确检验比较频率。细胞毒素活性比较采用Spearman秩校正检验。结果:72株菌株中有61株(84.7%)检测到空泡细胞毒素活性,但PU与胃炎菌株的空泡细胞毒素活性(83.7% vs 86.2%, P = 0.821)和滴度(4.4 +/- 3.8 vs 4.2 +/- 4.1, P = 0.876)差异无统计学意义。表达两种或两种以上Lewis抗原(Le(x)、Le(y)、Le(a)和Le(b))的PU菌株明显多于慢性胃炎患者(90.7% vs 65.5%, P = 0.029)。43株PU患者血清babA2阳性17株(39.5%),29株胃炎患者血清babA2阳性11株(38.5%)(P = 0.924)。表达两种或两种以上Lewis抗原和少于两种抗原的菌株的空泡细胞毒素活性和滴度无显著差异(84.5% vs 85.7%, P = 1.000;4.4 +/- 4.2 vs 4.3 +/- 3.2, P = 0.965)。72株幽门螺杆菌中,babA2阳性28株,细胞毒性24株,babA2阴性44株37株(P = 1.000)。结论:目前的研究表明,PU与中国人群幽门螺杆菌中Lewis抗原表达增加有关,而与空泡细胞毒素产生或babA2的存在无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association of peptic ulcer with increased expression of Lewis antigens, but not vacuolating cytotoxin activity or babA2 gene status, in Helicobacter pylori strains from China.

Objective: Controversies exist regarding the virulence factors, such as vacA, babA2 and Lewis blood group antigens, of Western and Asian strains of Helicobacter pylori. The aim of the present study was to determine the significance of these potential virulence factors in the Chinese population.

Methods: Seventy-two strains of H. pylori isolated from patients in Zhengzhou, China, including 43 cases of peptic ulcer (PU) and 29 cases of chronic gastritis, were determined. Vacuolating cytotoxin assay was performed by HeLa cells. The expression of Le blood group antigens (Le(a), Le(b), Le(x) and Le(y)) was performed by enzyme-linked immunosorbent assay (ELISA). babA2 gene was identified by polymerase chain reaction. Frequencies were compared using two-tailed Fisher's exact test. Cytotoxin activities were compared using Spearman's rank correction test.

Results: Vacuolating cytotoxin activity was detected in 61 of the 72 strains (84.7%), but there was no significant difference in vacuolating cytotoxin activity (83.7% vs 86.2%, P = 0.821) or titer (4.4 +/- 3.8 vs 4.2 +/- 4.1, P = 0.876) between the PU and gastritis strains. Significantly more PU strains expressed two or more Lewis antigens (Le(x), Le(y), Le(a) or Le(b)) than strains from the chronic gastritis patients (90.7% vs 65.5%, P = 0.029). Of the 43 strains from PU patients, 17 (39.5%) were positive for babA2, compared with 11 (38.5%) of the 29 strains from gastritis patients (P = 0.924). There was no significant difference in the vacuolating cytotoxin activity or titer between strains expressing two or more Lewis antigens and less than two antigens (84.5% vs 85.7%, P = 1.000; 4.4 +/- 4.2 vs 4.3 +/- 3.2, P = 0.965). Of the 72 H. pylori strains, 28 were babA2 positive, of which 24 were cytotoxic, compared with 37 of 44 babA2-negative strains (P = 1.000).

Conclusion: The present study suggests that PU is associated with increased Lewis antigen expression, but not vacuolating cytotoxin production or the presence of babA2, in the H. pylori strains in the Chinese population.

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