含有牛蒡燕麦胶体提取物的喷雾在体外皮肤重建模型中的愈合效果。

S Boisnic, M C Branchet, V Ermosilla
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摘要

通过重建皮肤模型,我们旨在评估含有Rhealba燕麦提取物的喷雾对新形成上皮的愈合速度和延伸的功效。首先在培养皿中通过将皮肤成纤维细胞与i型胶原蛋白结合制成真皮等量物,然后将穿孔活检作为表皮细胞的来源植入该真皮等量物上,形成多层表皮。将含有Rhealba燕麦提取物的喷雾添加到培养基中,通过免疫组织化学分析有丝分裂活性(5-溴-2'-脱氧尿苷[BrDu]结合)来评估表皮生长情况。与未治疗的重建皮肤相比,在22天内对新上皮的延伸进行组织学评估。第12天,BrDu阳性基底细胞在喷雾处理后为16%,而未处理的重建皮肤为4.2% (p < 0.05)。在第12天和第22天的表皮分化过程中,我们观察到在喷雾处理16天和18天后细胞上皮层的数量显著增加。此外,喷雾处理后第16天和第18天,再上皮化的延长时间也显著增加。总之,我们的研究结果表明,含有牛蒡提取物的喷雾对新形成的上皮细胞的再上皮化有积极的调节作用,并显示出愈合效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Healing effect of a spray containing Rhealba oat colloidal extract in an in vitro reconstitution model of skin.

By using a reconstituted skin model, we aimed to evaluate the efficacy of a spray containing Rhealba oat extract on the rapidity of healing and the extension of the newly formed epithelium. A dermal equivalent was first made in a petri dish by combining skin fibroblasts with collagen type I. Then a punch biopsy as a source of epidermal cells was implanted on this dermal equivalent, where a multilayered epidermis developed. The spray containing Rhealba oat extract was added to the culture medium to evaluate epidermal growth by immunohistochemical analysis of mitotic activity (5-bromo-2'-deoxyuridine [BrDu] incorporation). The extension of the neoepithelium in comparison with untreated reconstituted skin over 22 days was evaluated histologically. On day 12, 16% of positive BrDu basal cells was detected after spray treatment in comparison with 4.2% positive cells in untreated reconstituted skin (p < 0.05). During epidermal differentiation between days 12 and 22, we observed a significant increase in the number of cellular epithelial layers after 16 and 18 days of spray treatment. Moreover the extension of re-epithelialization was also significantly increased after spray treatment on days 16 and 18. In conclusion, our results demonstrate a positive modulation of re-epithelialization on a newly formed epithelium by a spray containing Rhealba oat extract displaying a healing effect.

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