[利用ISSR标记研究水稻早期稳定性]。

Li-Jun Zhou, Ai-Xian Li, Xian-Jun Wu, Shi-Gui Li
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引用次数: 0

摘要

在2个早稳定稻(Oryza sativa L.)与4个品种杂交的F2群体中,记录到8个统一品系。遗传分析表明,在同一组合的F2群体中,既有具有统一农艺性状的统一菌株,也有以孟德尔方式分离的分离菌株。用26条ISSR引物对F2均匀菌株进行标记后,得到4种类型的标记带:ⅰ型,有母系标记带,没有父系标记带;II型,存在父亲标记带,不存在母亲标记带;III型,部分母系和父系标记带存在,其余不存在;IV型出现新的重组标记带,母系和父系标记带均不存在。分离菌株存在杂合性标记带,存在母系和父系标记带。根据900号ISSR标记引物扩增出的2000 bp标记带,可以将水稻的均匀菌株和正常菌株分为两类。该结果可为水稻早稳遗传机制的研究提供实验依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Study on early stability by ISSR markers in rice].

In the F2 population crossed from two early stability rice (Oryza sativa L.) with four cultivars, eight uniform strains were recorded. Genetic analysis showed that both uniform strains with uniform agronomic characteristics and segregated strains segregating in Mendelian manner were observed in F2 population of the same combination. Four types of marker-bands were obtained after the F2 uniform strains were marked by 26 ISSR primers:t ype I, maternal marker bands present, and paternal marker bands absent; type II, paternal marker bands present, and maternal marker bands absent; type III, parts of maternal and paternal marker bands present, and the others absent; type IV, new recombined marker bands appeared, and all maternal and paternal marker bands absent. But segregating strains showed heterzygosity marker bands, maternal and paternal marker bands being present. The uniform strains and normal strains in rice might be grouped into two classes on the basis of the 2000 bp marker band amplified by the ISSR marker primers No. 900. This result would provide experimental basis for the study on genetic mechanism of early stability in rice.

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