日本七鳃鳗口腺表达序列标签(est)分析。

Qi Gao, Yue Pang, Yu Wu, Fei Ma, Qing-Wei Li
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引用次数: 0

摘要

以日本七鳃鳗(Lampetra japonica)口腔腺为样本,构建了2.1 × 10(6) pfu/mL的cDNA文库。随机选择克隆进行测序,得到插入片段长度大于100 bp且色谱图良好的克隆1 323个。使用BlastX和BlastN程序,我们发现653条ESTs(49.36%)与NCBI蛋白或核苷酸数据库中的已知序列具有显著的同源性,其中328条ESTs与已知的鼠足虫科动物序列同源。这些ESTs被划分为11个不同的功能类别,其中与蛋白质合成相关的比例最高。在1323条ESTs中,547条序列经软件比对,同源性至少在20个碱基对以上达到90%以上的阈值,共组装出162条非冗余contigs。8个全长cdna具有完整的开放阅读框。进一步研究该文库中的est,将有助于在分子水平上阐明日本七鳃鳗口腺的组成成分,并进一步了解这些蛋白的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Expressed sequence tags (ESTs) analysis of the oral gland of Lampetra japonica].

A cDNA library (2.1 x 10(6) pfu/mL) was constructed from the oral gland of Lampetra japonica. After random selection of clones for sequencing, 1 323 clones with inserts longer than 100 bp and with good chromato-grams were obtained. Using BlastX and BlastN programs, We found 653 ESTs (49.36%) that shared significant homology with known sequences in protein or nucleotide databases of NCBI, including 328 ESTs homologous to known sequences of Petromazonidae animals. These ESTs were classified into 11 different functional categories,the highest proportion of which was related to protein synthesis. Out of the 1323 ESTs, 162 non-redundant contigs were assembled from 547 sequences after alignment using software at the threshold of more than 90% homology over a minimum of 20 base pairs. There were eight full-length cDNAs with complete open reading frame. Further studies on the ESTs in the library may be helpful to elucidate the components of Lampetra japonica oral gland and to understand the function of those proteins at the molecular level.

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