{"title":"缺水对家鸡水通道蛋白4 (AQP4) mRNA表达的影响","authors":"Noboru Saito, Hidehiro Ikegami, Kiyoshi Shimada","doi":"10.1016/j.molbrainres.2005.09.005","DOIUrl":null,"url":null,"abstract":"<div><p>Aquaporin (AQP) 4 is a member of the AQP<span><span> gene family of water-selective transport proteins. We studied the effect of water deprivation on AQP4 gene expression in chickens. The </span>nucleotide sequence<span> of a chicken aquaporin 4 (AQP4) cDNA that encodes a protein<span><span> of 335 amino acids showed high homology to mammalian AQP4. Using Northern blotting analysis, AQP4 mRNA in chickens was observed as a band of approximately 5.5 kb in several tissues in addition to the hypothalamus, proventriculus, kidney, and breast muscle. Quantitative analysis by real-time RT-PCR analysis showed that the mRNA expression of AQP4 in the hypothalamus significantly increased after dehydration. On the other hand, the mRNA expression of AQP4 in the kidney significantly decreased after dehydration. This suggests that AQP4 may play a pivotal role in </span>osmoregulation in the chicken brain.</span></span></span></p></div>","PeriodicalId":100932,"journal":{"name":"Molecular Brain Research","volume":"141 2","pages":"Pages 193-197"},"PeriodicalIF":0.0000,"publicationDate":"2005-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.005","citationCount":"21","resultStr":"{\"title\":\"Effect of water deprivation on aquaporin 4 (AQP4) mRNA expression in chickens (Gallus domesticus)\",\"authors\":\"Noboru Saito, Hidehiro Ikegami, Kiyoshi Shimada\",\"doi\":\"10.1016/j.molbrainres.2005.09.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Aquaporin (AQP) 4 is a member of the AQP<span><span> gene family of water-selective transport proteins. We studied the effect of water deprivation on AQP4 gene expression in chickens. The </span>nucleotide sequence<span> of a chicken aquaporin 4 (AQP4) cDNA that encodes a protein<span><span> of 335 amino acids showed high homology to mammalian AQP4. Using Northern blotting analysis, AQP4 mRNA in chickens was observed as a band of approximately 5.5 kb in several tissues in addition to the hypothalamus, proventriculus, kidney, and breast muscle. Quantitative analysis by real-time RT-PCR analysis showed that the mRNA expression of AQP4 in the hypothalamus significantly increased after dehydration. On the other hand, the mRNA expression of AQP4 in the kidney significantly decreased after dehydration. This suggests that AQP4 may play a pivotal role in </span>osmoregulation in the chicken brain.</span></span></span></p></div>\",\"PeriodicalId\":100932,\"journal\":{\"name\":\"Molecular Brain Research\",\"volume\":\"141 2\",\"pages\":\"Pages 193-197\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-11-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.molbrainres.2005.09.005\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Brain Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0169328X05003633\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Brain Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0169328X05003633","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of water deprivation on aquaporin 4 (AQP4) mRNA expression in chickens (Gallus domesticus)
Aquaporin (AQP) 4 is a member of the AQP gene family of water-selective transport proteins. We studied the effect of water deprivation on AQP4 gene expression in chickens. The nucleotide sequence of a chicken aquaporin 4 (AQP4) cDNA that encodes a protein of 335 amino acids showed high homology to mammalian AQP4. Using Northern blotting analysis, AQP4 mRNA in chickens was observed as a band of approximately 5.5 kb in several tissues in addition to the hypothalamus, proventriculus, kidney, and breast muscle. Quantitative analysis by real-time RT-PCR analysis showed that the mRNA expression of AQP4 in the hypothalamus significantly increased after dehydration. On the other hand, the mRNA expression of AQP4 in the kidney significantly decreased after dehydration. This suggests that AQP4 may play a pivotal role in osmoregulation in the chicken brain.
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