miR-369-3p作为肝细胞癌的预后因子，调控其癌变进程。

IF 1.7 4区医学 Q3 PHARMACOLOGY & PHARMACY

Personalized medicine Pub Date : 2021-07-01 Epub Date: 2021-04-01 DOI:10.2217/pme-2020-0012

Can Chen, Yi Zong, Jiaojiao Tang, Ruisheng Ke, Lizhi Lv, Mengchao Wu, Junhua Lu

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引用次数: 2

摘要

背景:本研究的目的是探讨miR-369-3p在肝细胞癌(HCC)中的作用。材料与方法:采用实时定量逆转录pcr法检测miR-369-3p的表达水平。通过细胞计数试剂盒-8和transwell检测，探讨miR-369-3p对HCC细胞增殖、迁移和侵袭的影响。结果:与正常对照相比，miR-369-3p在HCC组织和细胞系中的表达分别下调。在体外，Hep 3B和Huh7细胞中过表达miR-369-3p可抑制细胞增殖、迁移和侵袭。SOX4是miR-369-3p的直接靶点。结论:我们的研究结果提示miR-369-3p可能通过靶向SOX4在HCC中发挥抑瘤作用。

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miR-369-3p serves as prognostic factor and regulates cancer progression of hepatocellular carcinoma.

Background: The aim of this study was to investigate the role of miR-369-3p in hepatocellular carcinoma (HCC). Materials & methods: The expression levels of miR-369-3p were detected using the quantitative real-time reverse transcription-PCR analysis. The cell counting kit-8 and transwell assays were used to explore the effects of miR-369-3p on cell proliferation, migration and invasion of HCC cells. Results: The miR-369-3p expression was downregulated in HCC tissues and cell lines, in comparison to the normal controls, respectively. In vitro, overexpression of miR-369-3p in Hep 3B and Huh7 cells inhibited cell proliferation, migration and invasion. SOX4 was a direct target of miR-369-3p. Conclusion: Our results suggested that miR-369-3p may be a tumor suppressor in HCC by targeting SOX4.

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来源期刊

Personalized medicine 医学-药学

CiteScore

3.30

自引率

4.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Personalized Medicine (ISSN 1741-0541) translates recent genomic, genetic and proteomic advances into the clinical context. The journal provides an integrated forum for all players involved - academic and clinical researchers, pharmaceutical companies, regulatory authorities, healthcare management organizations, patient organizations and others in the healthcare community. Personalized Medicine assists these parties to shape thefuture of medicine by providing a platform for expert commentary and analysis. The journal addresses scientific, commercial and policy issues in the field of precision medicine and includes news and views, current awareness regarding new biomarkers, concise commentary and analysis, reports from the conference circuit and full review articles.