体区对绒猴毛发皮质醇浓度的影响。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Comparative medicine Pub Date : 2021-04-01 Epub Date: 2021-03-22 DOI:10.30802/AALAS-CM-20-000091
Evelyn E Bartling-John, Kimberley A Phillips
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引用次数: 1

摘要

普通狨猴(Callithrix jacchus)由于其适应性、生理特性和易于实验操作的特点,是研究神经科学和衰老生物学影响的有价值的研究模型。毛发中皮质醇的量化提供了一种无创的、回顾性的下丘脑-垂体-肾上腺(HPA)轴活性生物标志物和动物健康信息,包括对环境和社会刺激的反应。为了获得有效和可靠的长期HPA活性测量,我们研究了毛中皮质醇浓度的变异性,这取决于狨猴的身体区域。在年度健康检查期间从9只成年普通狨猴的背部和尾部收集毛发(雄性n = 3;女性n = 6),这些样本通过甲醇提取和酶免疫分析法分析皮质醇。我们发现毛发皮质醇浓度在尾部和背部区域之间存在差异,尾部样本的皮质醇浓度明显较高。这些结果表明,毛发皮质醇浓度的个体内和个体间比较应该使用从狨猴同一身体区域获得的毛发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Body Region on Hair Cortisol Concentration in Common Marmosets (Callithrix jacchus).

Common marmosets (Callithrix jacchus) are a valuable research model for the study of neuroscience and the biologic impact of aging due to their adaptivity, physiologic characteristics, and ease of handling for experimental manipulations. Quantification of cortisol in hair provides a noninvasive, retrospective biomarker of hypothalamics-pituitary-adrenal (HPA) axis activity and information on animal wellbeing, including responses to environmental and social stimuli. To obtain valid and reliable measurements of long-term HPA activity, we investigated the variability of cortisol concentration in the hair depending on the body region of marmosets. Hair was collected from the back and tail of 9 adult common marmosets during annual health screenings (male n = 3; female n = 6) and these samples were analyzed for cortisol via methanol extraction and enzyme immunoassay. We found that hair cortisol concentration differed between the tail and back regions, with the tail samples having a significantly higher cortisol concentration. These results indicate intraindividual and interindividual comparisons of hair cortisol concentration should use hair obtained from the same body region in marmosets.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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