小麦雄性不育基因同源序列的分离、鉴定及表达分析

Jun-Fang Chen, Zheng-Long Ren, Xiu-Ying Kong, Jia-Jie Wu, Rong-Hua Zhou, Ji-Zeng Jia
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引用次数: 0

摘要

利用拟南芥和水稻MS2基因保守结构域设计的一对退化引物在小麦雄性不育和可育花药中扩增出一个134 bp的片段,并利用该134 bp的片段通过硅克隆扩增出一个1604 bp的雄性不育基因同源序列。雄性不育基因同源序列编码的氨基酸包含一个200个氨基酸的雄性不育保守结构域,该序列仅在小麦雄性可育花药中表达;在雄性不育花药、根和叶中不表达。本研究表明,克隆的雄性不育同源序列是小麦花药发育所特有的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Isolation, characterization and expression analysis of male sterility gene homology sequence in wheat].

One 134 bp fragment was amplified in anthers of male sterile and fertile wheat using one pair of degenerated primer designed based on the conserved domain of MS2 gene in Arabidopsis thaliana and Oryza sativa, and one 1604 bp male sterility gene homology sequence was extended by in silico cloning based on the 134 bp fragment. The amino acids encoded by the male sterility gene homology sequence include a 200 amino acid conserved domain of male sterility, and this sequence expressed only in wheat male fertile anthers; no expression in male sterile anthers, roots and leaves. This research demonstrated that the cloned male sterile homology sequence is specific to wheat anther development.

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