B Brando, A Longo, B Beltrami, D Passoni, R Verna, F Licastro, M M Corsi
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引用次数: 0
摘要
一种新的方法来测量端粒长度在人口唐氏综合症患者年龄18-60提出。该方法基于流式细胞术和全细胞定量荧光原位杂交(flow- fish)。已经描述了至少三种测量端粒重复序列长度的方法:(i) Southern blot分析,以及使用(ii)数字荧光显微镜(Q-FISH)或(iii)流式细胞术(flow-FISH)的定量FISH。Southern blot分析和Q-FISH都有特定的局限性,而且耗时,而flow-FISH需要的细胞相对较少(1.5-2.5 x 106),可以在24-48小时内完成。该方法可用于快速测定年龄相关疾病(如唐氏综合征、阿尔茨海默病和Werner综合征)患者的有核血细胞亚群的端粒长度。
Determination of telomere length by flow-fluorescence in situ hybridization in Down's syndrome patients.
A new method for measuring telomere length in a population of Down's syndrome patients aged 18-60 years old is presented. The method is based on flow cytometry and quantitative fluorescence in situ hybridization (flow-FISH) on whole cells. At least three methods for measuring the length of telomere repeats have been described: (i) Southern blot analysis, and quantitative FISH using either (ii) digital fluorescence microscopy (Q-FISH) or (iii) flow cytometry (flow-FISH). Both Southern blot analysis and Q-FISH have specific limitations and are time-consuming, whereas flow-FISH needed relatively few cells (1.5-2.5 x 106) and could be completed in 24-48 h. The method can be used to rapidly determine telomere length in subsets of nucleated blood cells from patients with age-related diseases such as Down's syndrome, Alzheimer's disease and Werner syndrome.