{"title":"用于分离和纯化蛋白质和肽的磁性技术。","authors":"Ivo Safarik, Mirka Safarikova","doi":"10.1186/1477-044X-2-7","DOIUrl":null,"url":null,"abstract":"<p><p>Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.</p>","PeriodicalId":8888,"journal":{"name":"Biomagnetic Research and Technology","volume":"2 1","pages":"7"},"PeriodicalIF":0.0000,"publicationDate":"2004-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544596/pdf/","citationCount":"0","resultStr":"{\"title\":\"Magnetic techniques for the isolation and purification of proteins and peptides.\",\"authors\":\"Ivo Safarik, Mirka Safarikova\",\"doi\":\"10.1186/1477-044X-2-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.</p>\",\"PeriodicalId\":8888,\"journal\":{\"name\":\"Biomagnetic Research and Technology\",\"volume\":\"2 1\",\"pages\":\"7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2004-11-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544596/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomagnetic Research and Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/1477-044X-2-7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomagnetic Research and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/1477-044X-2-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Magnetic techniques for the isolation and purification of proteins and peptides.
Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.