排卵后衰老诱导小鼠透明带的结构变化。

H Díaz, P Esponda
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引用次数: 0

摘要

分析了排卵后不久采集的小鼠卵母细胞和20 h后采集的小鼠卵母细胞的透明带(ZP)结构。采用传统的电子显微镜方法和酒精PTA染色程序,优先对比赖氨酸丰富的蛋白质。衰老卵母细胞的ZP显示出一些结构变化,特别是在PTA程序后观察到的。在82.14%的衰老卵母细胞中,ZP明显由两个不同的区域组成:内致密区和外低密度区。ZP呈纤维带状结构,纤维丝在内外区平行排列。体外受精分析表明,衰老配子达到双细胞胚胎期的比例仅为16.85%,而新鲜排卵的配子达到66.93%。未受精的卵母细胞显示精子未穿透ZP。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Postovulatory ageing induces structural changes in the mouse zona pellucida.

The structure of the zona pellucida (ZP) was analyzed in mouse oocytes collected soon after ovulation and in others retrieved 20 h after. The conventional methods for electron microscopy and the alcoholic PTA staining procedure which preferentially contrasts lysine-rich proteins were employed. The ZP of aged oocytes showed several structural changes which were particularly observed after using the PTA procedure. In 82.14% of the aged oocytes the ZP appeared clearly composed of two different regions: an inner dense and an outer of low density. The ZP showed a fibrillar banded structure with a parallel arrangement of fibrillar threads in both the outer and inner regions. The in vitro fertilization analysis showed that only 16.85% of the aged gametes attained the two cell embryo stage in comparision to 66.93% shown by the freshly ovulated eggs. The non-fertilized oocytes showed that no sperm penetration through the ZP occurred.

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